Rodríguez-Hernández J A, Martín-Barrientos J, Sánchez-Bernal C, Cabezas J A
Int J Biochem. 1987;19(5):449-53. doi: 10.1016/0020-711x(87)90066-8.
The separation of four different hexosaminidase forms from embryonic chicken brain (16-day-old) has been performed by ion-exchange chromatography. Two different DEAE-cellulose columns have been used: a first one at pH 7.2 and a second one at pH 6.0. Km and Vmax values were estimated from the Lineweaver-Burk or Dixon plots and ki from the Dixon plots, using N-acetyl-D-glucosamine or N-acetyl-D-galactosamine as inhibitors. In both cases we found a kind of competitive inhibition in which Lineweaver-Burk and Dixon plots curve downwards.
通过离子交换色谱法从16日龄胚胎鸡脑中分离出四种不同形式的己糖胺酶。使用了两种不同的DEAE - 纤维素柱：第一个在pH 7.2，第二个在pH 6.0。使用N - 乙酰 - D - 葡萄糖胺或N - 乙酰 - D - 半乳糖胺作为抑制剂，根据Lineweaver - Burk或Dixon图估算Km和Vmax值，并根据Dixon图估算Ki值。在这两种情况下，我们都发现了一种竞争性抑制，其中Lineweaver - Burk和Dixon图向下弯曲。
