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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Sanchez-Bernal C, Martin-Barrientos J, Cabezas J A

Int J Biochem. 1983;15(5):703-8. doi: 10.1016/0020-711x(83)90195-7.

A "neutral" hexosaminidase has been separated from other hexosaminidase forms (I and II) by DEAE-cellulose chromatography and characterized in embryonic (16-days old) and 1-day old chicken brains. 2. Its properties differ from those of the forms I and II. It has optimum activity at about pH 6.0 and can be eluted from DEAE-cellulose with 0.25 M KCl only. 3. It has no N-acetylgalactosaminidase activity and cannot be successfully detected after isoelectric focusing since it is very acidic and completely unstable below pH 5.0. 4. "Neutral" hexosaminidase is heat-stable at pH 6.0 and is inhibited by chloride. 5. These properties, very different from those of forms I and II, suggest that this "neutral" form of hexosaminidase would be very similar to known hexosaminidase C separated from other materials. 6. We have found no significant differences for the above-mentioned three forms in chick embryos (16-days old) in comparison with those from 1-day old chicken.

通过DEAE - 纤维素色谱法已从其他己糖胺酶形式（I和II）中分离出一种“中性”己糖胺酶，并对16日龄胚胎和1日龄鸡脑进行了特性鉴定。2. 其特性与形式I和II不同。它在约pH 6.0时具有最佳活性，并且只能用0.25 M KCl从DEAE - 纤维素中洗脱。3. 它没有N - 乙酰半乳糖胺酶活性，在等电聚焦后无法成功检测到，因为它非常酸性且在pH 5.0以下完全不稳定。4. “中性”己糖胺酶在pH 6.0时热稳定且受氯离子抑制。5. 这些特性与形式I和II的特性非常不同，表明这种“中性”形式的己糖胺酶与从其他材料中分离出的已知己糖胺酶C非常相似。6. 我们发现，与1日龄鸡相比，上述三种形式在16日龄鸡胚胎中没有显著差异。

Sanchez-Bernal C, Martin-Barrientos J, Cabezas J A

Int J Biochem. 1983;15(5):703-8. doi: 10.1016/0020-711x(83)90195-7.

A "neutral" hexosaminidase has been separated from other hexosaminidase forms (I and II) by DEAE-cellulose chromatography and characterized in embryonic (16-days old) and 1-day old chicken brains. 2. Its properties differ from those of the forms I and II. It has optimum activity at about pH 6.0 and can be eluted from DEAE-cellulose with 0.25 M KCl only. 3. It has no N-acetylgalactosaminidase activity and cannot be successfully detected after isoelectric focusing since it is very acidic and completely unstable below pH 5.0. 4. "Neutral" hexosaminidase is heat-stable at pH 6.0 and is inhibited by chloride. 5. These properties, very different from those of forms I and II, suggest that this "neutral" form of hexosaminidase would be very similar to known hexosaminidase C separated from other materials. 6. We have found no significant differences for the above-mentioned three forms in chick embryos (16-days old) in comparison with those from 1-day old chicken.

通过DEAE - 纤维素色谱法已从其他己糖胺酶形式（I和II）中分离出一种“中性”己糖胺酶，并对16日龄胚胎和1日龄鸡脑进行了特性鉴定。2. 其特性与形式I和II不同。它在约pH 6.0时具有最佳活性，并且只能用0.25 M KCl从DEAE - 纤维素中洗脱。3. 它没有N - 乙酰半乳糖胺酶活性，在等电聚焦后无法成功检测到，因为它非常酸性且在pH 5.0以下完全不稳定。4. “中性”己糖胺酶在pH 6.0时热稳定且受氯离子抑制。5. 这些特性与形式I和II的特性非常不同，表明这种“中性”形式的己糖胺酶与从其他材料中分离出的已知己糖胺酶C非常相似。6. 我们发现，与1日龄鸡相比，上述三种形式在16日龄鸡胚胎中没有显著差异。