Doğan Mustafa, Çelikkol Aliye, Yılmaz Berna, Erdem İlknur
Tekirdağ Namık Kemal University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Tekirdağ, Türkiye.
Tekirdağ Namık Kemal Üniversitesi Faculty of Medicine, Department of Medical Biochemistry, Tekirdağ, Türkiye.
Mikrobiyol Bul. 2022 Jul;56(3):545-552. doi: 10.5578/mb.20229713.
The coronavirus disease-2019 (COVID-19) pandemic continues to threaten the lives of millions of people. Viral shedding through the respiratory tract is the main risk factor for the transmission of the severe acute respiratory syndrome-2 (SARS-CoV-2) virus from sick individuals to healthy individuals. In this study, we aimed to investigate the viral clearance (VC) time in PCR tests of COVID-19 patients and the possible factors affecting this time. Seventy patients older than 18 years of age whose presence of SARS-CoV-2 virus was proven by real-time polymerase chain reaction (Rt-PCR) in nasopharyngeal swab samples were included in the study. The presence of SARS-CoV-2 RNA was investigated by RT-PCR in nasopharyngeal swab samples at 48-72 hour intervals, five days after the initial diagnosis. Demographic , physical examination, laboratory test, computed tomography (CT) results, concomitant diseases, and duration of VC were recorded. Of the cases, 41 were female and 29 were male. The mean age was 45.8 ± 19.2 years. According to the CT results, in the group with no involvement, local involvement and widespread involvement, the duration of VC was 9.66 ± 5.91 days, 9.99 ± 4.68 days, and 10.94 ± 5.34 days, respectively (p> 0.05). While the duration of VC was determined as 8.93 ± 4.33 days in the group without comorbidity, this period was found to be 12.26 ± 5.69 days (p= 0.025) in the group with the comorbidity. It was determined that the duration of VC was 9.55 ± 6.37 days in women and 9.20 ± 7.22 days in men (p= 0.040). The duration of VC was found to be 10.18 ± 7.1 days in patients over 50 years of age and 8.87 ± 5.15 days under 50 years of age (p= 0.03). A significant correlation was found between the laboratory test lactate dehydrogenase level and VC duration (p= 0.007). However, a significant relationship could not be established between other laboratory test results and the duration of VC. In this retrospective observational study, the relationship between viral clearance duration in Rt-PCR and gender, age, CT results, comorbidities and laboratory results in nasopharyngeal swab samples was investigated and it was determined that the duration of VC was significantly prolonged in case of female gender, being over 50 years old and having a comorbid disease. The results obtained may contribute to predict the isolation times of the patients and to reveal the factors that may affect viral shedding.
2019冠状病毒病（COVID-19）大流行继续威胁着数百万人的生命。通过呼吸道进行病毒传播是严重急性呼吸综合征冠状病毒2（SARS-CoV-2）从患者传播至健康个体的主要风险因素。在本研究中，我们旨在调查COVID-19患者PCR检测中的病毒清除（VC）时间以及影响该时间的可能因素。本研究纳入了70名18岁以上的患者，其鼻咽拭子样本经实时聚合酶链反应（Rt-PCR）证实存在SARS-CoV-2病毒。在初次诊断五天后，每隔48 - 72小时通过RT-PCR检测鼻咽拭子样本中SARS-CoV-2 RNA的存在情况。记录人口统计学信息、体格检查、实验室检查、计算机断层扫描（CT）结果、合并症以及VC持续时间。病例中，女性41例，男性29例。平均年龄为45.8±19.2岁。根据CT结果，在无受累、局部受累和广泛受累组中，VC持续时间分别为9.66±5.91天、9.99±4.68天和10.94±5.34天（p>0.05）。在无合并症组中，VC持续时间确定为8.93±4.33天，而在有合并症组中，这一时期为12.26±5.69天（p = 0.025）。确定女性的VC持续时间为9.55±6.37天，男性为9.20±7.22天（p = 0.040）。50岁以上患者的VC持续时间为10.18±7.1天，50岁以下患者为8.87±5.15天（p = 0.03）。发现实验室检测乳酸脱氢酶水平与VC持续时间之间存在显著相关性（p = 0.007）。然而，其他实验室检测结果与VC持续时间之间未建立显著关系。在这项回顾性观察研究中，调查了Rt-PCR中的病毒清除持续时间与性别、年龄、CT结果、合并症以及鼻咽拭子样本中的实验室结果之间的关系，确定在女性、50岁以上以及患有合并症的情况下，VC持续时间显著延长。所获得的结果可能有助于预测患者的隔离时间，并揭示可能影响病毒传播的因素。
