Dept. of Chemistry, University of Utah, 315 S. 1400 East, Salt Lake City, Utah 84112-0850, United States.
Org Lett. 2022 Aug 26;24(33):6182-6185. doi: 10.1021/acs.orglett.2c02427. Epub 2022 Aug 12.
In RNA, pseudouridine () and 5-methylcytidine () are located by their differential reactions with NaHSO at pH 5. The pyrimidines were allowed to react with NaHSO, NaN, NaCN, or NaSCN at pH 5 to find that NaHSO was unique in achieving quantitative yields. Pseudouridine reaction selectivity with NaHSO was found at pH 7 supported by the reaction rate constants. The derivative N1-methylpseudouridine found in mRNA vaccines reacts similarly with bisulfite to yield ribose adducts.
在 RNA 中,假尿嘧啶 () 和 5-甲基胞嘧啶 () 是通过它们在 pH 5 下与 NaHSO 的差异反应来定位的。嘧啶类物质被允许在 pH 5 下与 NaHSO、NaN、NaCN 或 NaSCN 反应,以发现只有 NaHSO 能实现定量产率。假尿嘧啶与 NaHSO 的反应选择性在 pH 7 下得到了反应速率常数的支持。mRNA 疫苗中发现的 N1-甲基假尿嘧啶衍生物与亚硫酸氢盐反应类似,生成核糖加合物。