Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent B-9052, Belgium.
VIB Center for Plant Systems Biology, Ghent B-9052, Belgium.
Sci Adv. 2022 Aug 12;8(32):eabo7737. doi: 10.1126/sciadv.abo7737.
The regenerative potential in response to wounding varies widely among species. Within the plant lineage, the liverwort displays an extraordinary regeneration capacity. However, its molecular pathways controlling the initial regeneration response are unknown. Here, we demonstrate that the Mp transcription factor gene is instantly activated after wounding and is essential for gemmaling regeneration following tissue incision. MpERF15 operates both upstream and downstream of the MpCOI1 oxylipin receptor by controlling the expression of oxylipin biosynthesis genes. The resulting rise in the oxylipin dinor-12-oxo-phytodienoic acid (dn-OPDA) levels results in an increase in gemma cell number and apical notch organogenesis, generating highly disorganized and compact thalli. Our data pinpoint Mp as a key factor activating an oxylipin biosynthesis amplification loop after wounding, which eventually results in reactivation of cell division and regeneration. We suggest that the genetic networks controlling oxylipin biosynthesis in response to wounding might have been reshuffled over evolution.
物种之间对创伤的再生潜力差异很大。在植物谱系中，地钱表现出非凡的再生能力。然而，控制其初始再生反应的分子途径尚不清楚。在这里，我们证明 Mp 转录因子基因在受伤后立即被激活，并且对于组织切口后的孢子体再生是必需的。MpERF15 通过控制类脂氧合酶生物合成基因的表达，在上游和下游都作用于 MpCOI1 类脂氧合酶受体。由此产生的类脂氧合酶二降-12-氧代-植二烯酸（dn-OPDA）水平的升高导致了芽胞细胞数量的增加和顶端切痕器官发生，产生了高度紊乱和密集的叶状体。我们的数据指出 Mp 是在受伤后激活类脂氧合酶生物合成放大环的关键因素，最终导致细胞分裂和再生的重新激活。我们认为，响应创伤的类脂氧合酶生物合成的遗传网络在进化过程中可能已经重新组合。
