Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 11031, Taiwan; Department of Medical Research, Taipei Medical University Hospital, Taipei City 11031, Taiwan; International Ph. D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 11031, Taiwan.
Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 11031, Taiwan; School of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 11031, Taiwan.
Colloids Surf B Biointerfaces. 2022 Oct;218:112717. doi: 10.1016/j.colsurfb.2022.112717. Epub 2022 Jul 22.
Surface topography-induced lineage commitment of human bone marrow stem cells (hBMSCs) has been reported. However, this effect on hBMSC differentiation toward retinal pigment epithelium (RPE)-like cells has not been explored. Herein, a family of cell culture substrates called binary colloidal crystals (BCCs) was used to stimulate hBMSCs into RPE-like cells without induction factors. Two BCCs, named SiPS (silica (Si)/polystyrene (PS)) and SiPSC (Si/carboxylated PS), having similar surface topographies but different surface chemistry was used for cell culture. The result showed that cell proliferation was no difference between the two BCCs and tissue culture polystyrene (TCPS) control. However, the cell attachment, spreading area, and aspect ratio between surfaces were significantly changed. For example, cells displayed more elongated on SiPS (aspect ratio 7.0) than those on SiPSC and TCPS (2.0). The size of focal adhesions on SiPSC (1.6 µm) was smaller than that on the TCPS (2.5 µm). qPCR results showed that hBMSCs expressed higher RPE progenitor genes (i.e., MITF and PAX6) on day 15, and mature RPE genes (i.e., CRALBP and RPE65) on day 30 on SiPS than TCPS. On the other hand, the expression of optical vesicle or neuroretina genes (i.e., MITF and VSX2) was upregulated on day 15 on SiPSC compared to the TCPS. This study reveals that hBMSCs could be modulated into different cell subtypes depending on the BCC combinations. This study shows the potential of BCCs in controlling stem cell differentiation.
已有研究报道，骨髓间充质干细胞（hBMSCs）的表面形貌可诱导其向谱系分化。然而，其对 hBMSC 向视网膜色素上皮（RPE）样细胞分化的影响尚未被探索。在此，我们使用了一种名为二元胶体晶体（BCC）的细胞培养基底来刺激 hBMSCs 分化为 RPE 样细胞，而无需诱导因子。两种 BCC，分别命名为 SiPS（二氧化硅（Si）/聚苯乙烯（PS））和 SiPSC（Si/羧化 PS），具有相似的表面形貌但表面化学性质不同，用于细胞培养。结果表明，两种 BCC 与组织培养聚苯乙烯（TCPS）对照相比，细胞增殖没有差异。然而，细胞附着、铺展面积和表面纵横比有显著变化。例如，细胞在 SiPS 上呈现更细长的形态（纵横比约为 7.0），而在 SiPSC 和 TCPS 上则较宽（约 2.0）。SiPSC 上的焦点黏附大小（约 1.6 µm）小于 TCPS 上的焦点黏附大小（约 2.5 µm）。qPCR 结果表明，hBMSCs 在第 15 天在 SiPS 上表达更高的 RPE 祖细胞基因（即 MITF 和 PAX6），在第 30 天在 SiPS 上表达更高的成熟 RPE 基因（即 CRALBP 和 RPE65），而在 TCPS 上表达较低。另一方面，SiPSC 上的光学泡或神经视网膜基因（即 MITF 和 VSX2）在第 15 天的表达上调高于 TCPS。本研究揭示了 hBMSCs 可以根据 BCC 组合被调节为不同的细胞亚型。本研究表明了 BCC 在控制干细胞分化方面的潜力。
