Wang Peng-Yuan, Thissen Helmut, Kingshott Peter
Department of Chemistry and Biotechnology, Swinburne University of Technology , Hawthorn, 3122 Victoria, Australia.
CSIRO Manufacturing , Bayview Avenue, Clayton, 3168 Victoria, Australia.
ACS Appl Mater Interfaces. 2016 Feb;8(7):4477-88. doi: 10.1021/acsami.5b12660. Epub 2016 Feb 9.
A new surface based on self-assembly of two colloids into well-defined nanostructures, so-called binary colloidal crystals (BCCs), was fabricated for stem cell culture. The facile fabrication process are able to cover large surface areas (>3 cm-diameter, i.e. > 7 cm(2)) with ordered surface nanotopographies that is often a challenge particularly in biomaterials science. From our library, four different combinations of BCCs were selected using mixtures of silica, polystyrene and poly(methyl methacrylate) particles with sizes in the range from 100 nm to 5 μm. Cell spreading, proliferation, and surface-induced lineage commitment of human adipose-derived stem cells (hADSCs) was studied using quantitative real time polymerase chain reaction (qRT-PCR) and immunostaining. The results showed that BCCs induced osteo- and chondro- but not adipo-gene expression in the absence of induction medium suggesting that the osteochondral lineage can be stimulated by the BCCs. When applying induction media, higher osteo- and chondro-gene expression on BCCs was found compared with tissue culture polystyrene (TCPS) and flat silica (Si) controls, respectively. Colony forming of chondrogenic hADSCs was found on BCCs and TCPS but not Si controls, suggesting that the differentiation of stem cells is surface-dependent. BCCs provide access to complex nanotopographies and chemistries, which can find applications in cell culture and regenerative medicine.
一种基于两种胶体自组装形成明确纳米结构的新型表面，即所谓的二元胶体晶体（BCCs），被制备用于干细胞培养。这种简便的制备过程能够用有序的表面纳米拓扑结构覆盖大面积（直径>3厘米，即>7平方厘米），这在生物材料科学中通常是一个挑战。从我们的文库中，使用尺寸范围为100纳米至5微米的二氧化硅、聚苯乙烯和聚（甲基丙烯酸甲酯）颗粒混合物，选择了四种不同组合的BCCs。使用定量实时聚合酶链反应（qRT-PCR）和免疫染色研究了人脂肪来源干细胞（hADSCs）的细胞铺展、增殖和表面诱导的谱系定向。结果表明，在没有诱导培养基的情况下，BCCs诱导了骨和软骨而非脂肪生成基因的表达，这表明BCCs可以刺激骨软骨谱系。当应用诱导培养基时，与组织培养聚苯乙烯（TCPS）和平坦二氧化硅（Si）对照相比，在BCCs上分别发现了更高的骨和软骨基因表达。在BCCs和TCPS上发现了软骨生成hADSCs的集落形成，但在Si对照上未发现，这表明干细胞的分化是表面依赖性的。BCCs提供了获得复杂纳米拓扑结构和化学性质的途径，可应用于细胞培养和再生医学。
