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自组装二元胶体晶体单层作为细胞培养底物

Self-assembled binary colloidal crystal monolayers as cell culture substrates.

作者信息

Wang Peng-Yuan, Pingle Hitesh, Koegler Peter, Thissen Helmut, Kingshott Peter

机构信息

Swinburne University of Technology, Industrial Research Institute Swinburne (IRIS), Department of Chemistry and Biotechnology, Hawthorn, 3122 VIC, Australia.

出版信息

J Mater Chem B. 2015 Mar 28;3(12):2545-2552. doi: 10.1039/c4tb02006e. Epub 2015 Feb 24.

Abstract

This study investigated the formation of self-assembled binary colloidal crystal (BCC) monolayers using evaporation induced confined area assembly (EICAA), and fabricated a family of various BCCs for cell culture. A library of various BCCs with different structures was established and it was demonstrated that after stabilisation the BCCs have potential to be used as substrates with well-ordered surface topographies and chemistries for manipulating cell-surface interactions. Three cell types including MG63 osteoblasts, L929 fibroblasts, and primary human adipose-derived stem cells (hADSCs) show different responses on selected surfaces (either between BCCs or BCCs vs. flat controls). In general, cell spreading was inhibited on BCCs due to surface topography. However, the chemical composition presented on the BCCs can compensate the topographic effect depending on what combination was used. The ordered topographic and heterogeneous chemical patterns provide a complexity of surface properties and have potential to be selectively modified with desired biomolecules for controlling biointerface interactions.

摘要

本研究利用蒸发诱导受限区域组装(EICAA)研究了自组装二元胶体晶体(BCC)单层的形成,并制备了一系列用于细胞培养的不同BCC。建立了具有不同结构的各种BCC文库,结果表明,稳定后的BCC有潜力用作具有有序表面形貌和化学性质的底物,用于操纵细胞-表面相互作用。包括MG63成骨细胞、L929成纤维细胞和原代人脂肪来源干细胞(hADSCs)在内的三种细胞类型在选定表面(BCC之间或BCC与平坦对照之间)表现出不同的反应。一般来说,由于表面形貌,细胞在BCC上的铺展受到抑制。然而,BCC上呈现的化学成分可以根据所使用的组合来补偿形貌效应。有序的形貌和异质化学模式提供了表面性质的复杂性,并且有潜力用所需的生物分子进行选择性修饰,以控制生物界面相互作用。

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