80 Hz but not 40 Hz, transcranial alternating current stimulation of 80 Hz over right intraparietal sulcus increases visuospatial working memory capacity.

Working memory (WM) is a complex cognitive function involved in the temporary storage and manipulation of information, which has been one of the target cognitive functions to be restored in neurorehabilitation. WM capacity is known to be proportional to the number of gamma cycles nested in a single theta cycle. Therefore, gamma-band transcranial alternating current stimulation (tACS) should be dependent of the stimulation frequency; however, the results of previous studies that employed 40 Hz tACS have not been consistent. The optimal locations and injection currents of multiple scalp electrodes were determined based on numerical simulations of electric field. Experiments were conducted with 20 healthy participants. The order of three stimulation conditions (40 Hz tACS, 80 Hz tACS, and sham stimulation) were randomized but counterbalanced. Visual hemifield-specific visual WM capacity was assessed using a delayed visual match to the sample task. High gamma tACS significantly increased WM capacity, while low gamma tACS had no significant effect. Notably, 80 Hz tACS increased WM capacity on both the left and right visual hemifields, while previous tACS studies only reported the effects of tACS on contralateral hemifields. This is the first study to investigate the frequency-dependent effect of gamma-band tACS on WM capacity. Our findings also suggest that high gamma tACS might influence not only WM capacity but also communication between interhemispheric cortical regions. It is expected that high gamma tACS could be a promising neurorehabilitation method to enhance higher-order cognitive functions with similar mechanisms.