van Putten A J, Jochems G J, de Lang R, Nijkamp H J
Gene. 1987;51(2-3):171-8. doi: 10.1016/0378-1119(87)90305-2.
Mobilization of the non-conjugative plasmid CloDF13 requires both gene products of a conjugative plasmid and CloDF13-encoded information. About 30% of the CloDF13 genome is involved in plasmid transfer. The CloDF13 mobilization region comprises sequences acting in cis (bom, basis of mobilization) as well as protein-coding sequences (mob). Here we present the nucleotide sequence of the genes encoding the CloDF13 mobilization proteins. We confirmed the previous genetic data that the plasmid CloDF13 encodes two proteins involved in plasmid mobilization. The information for these proteins, designated B and C having Mrs of 57,890 and 15,870, respectively, is located within one operon directed by a promoter at 94% of the CloDF13 genome. The gene encoding the smaller protein is located distally within this operon. Transcription proceeds counter-clockwise and is terminated beyond gene C, although it can not be excluded that attenuation of the transcript occurs in the intergenic region. The role of the CloDF13 mobilization proteins in plasmid transfer will be discussed.
非接合性质粒CloDF13的转移需要一个接合性质粒的两个基因产物以及CloDF13编码的信息。约30%的CloDF13基因组参与质粒转移。CloDF13转移区域包含顺式作用序列（bom，转移基础）以及蛋白质编码序列（mob）。本文给出了编码CloDF13转移蛋白的基因的核苷酸序列。我们证实了先前的遗传学数据，即质粒CloDF13编码两种参与质粒转移的蛋白质。这些蛋白质分别命名为B和C，相对分子质量分别为57,890和15,870，其信息位于由CloDF13基因组94%处的一个启动子指导的一个操纵子内。编码较小蛋白质的基因位于该操纵子的远端。转录按逆时针方向进行，并在基因C之后终止，不过不能排除转录本在基因间区域发生衰减的可能性。将讨论CloDF13转移蛋白在质粒转移中的作用。
