Nijkamp H J, de Lang R, Stuitje A R, van den Elzen P J, Veltkamp E, van Putten A J
Plasmid. 1986 Sep;16(2):135-60. doi: 10.1016/0147-619x(86)90072-7.
The complete nucleotide sequence of the bacteriocinogenic plasmid CloDF13 has been determined. The plasmid consists of 9957 base pairs (molecular weight 6.64 X 10(6] with a GC content of 54.4%. At this moment 16 identified biological functions can be assigned to the primary structure of the CloDF13 DNA. The functions include those of eight protein encoding genes, two untranslated RNA species, and six DNA sites. We discuss these functions in relation to the structure of CloDF13 DNA. For convenience we have divided the CloDF13 genome into five defined regions: region I (origin of vegetative replication, priming and control of replication, type I incompatibility), region II (cloacin DF13, cloacin immunity, cloacin release, cloacin operon control), region III (double-stranded DNA-phage interaction, type II incompatibility, multimer resolution), region IV (inhibition of male specific RNA phages and transfer of Flac), and region V (mobility proteins, basis of mobility).
致育因子质粒CloDF13的完整核苷酸序列已被测定。该质粒由9957个碱基对组成（分子量为6.64×10⁶），GC含量为54.4%。目前，CloDF13 DNA的一级结构可赋予16种已确定的生物学功能。这些功能包括8个蛋白质编码基因、2种非翻译RNA种类以及6个DNA位点的功能。我们结合CloDF13 DNA的结构来讨论这些功能。为方便起见，我们将CloDF13基因组划分为五个明确的区域：区域I（营养复制起点、复制引发与控制、I型不相容性），区域II（杀梭菌素DF13、杀梭菌素免疫、杀梭菌素释放、杀梭菌素操纵子控制），区域III（双链DNA噬菌体相互作用、II型不相容性、多聚体解析），区域IV（抑制雄性特异性RNA噬菌体和Flac转移），以及区域V（迁移蛋白、迁移基础）。
