Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, PR China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Biol Macromol. 2022 Oct 31;219:1034-1046. doi: 10.1016/j.ijbiomac.2022.08.035. Epub 2022 Aug 10.
Chinese yam polysaccharides (CYP) exhibit superior adjuvant activity and modulate the immune response, but the low bioavailability limits their clinical application. Pickering emulsions have been proven as an efficient vaccine delivery system to enhance the immune response. Here, we used the Chinese yam polysaccharides PLGA-stabilized Pickering emulsion adjuvant system (CYP-PPAS) loaded with Porcine circovirus 2 as a vaccine and focused on investigating its adjuvant activity on humoral and cellular immunity in mice. The CYP-PPAS increased PCV-2 antigen loading efficiency and showed a high antigen uptake efficiency by macrophages in vitro. In vivo, CYP-PPAS significantly facilitated DCs maturation in draining lymph nodes than CYP or PPAS alone group. The CYP-PPAS also induced an increased proliferation index and a CD4/CD8 ratio. Meanwhile, in contrast to the CYP and PPAS groups, CYP-PPAS elicited a stronger anti-PCV-2 IgG and mixed Th1/Th2 immune response. Specifically, the CYP-PPAS group displayed the high expression of CD107a, FasL, and Granzyme B secretion to augment a strong cytotoxic lymphocyte response. Overall, the CYP-PPAS was a successful adjuvant system for promoting humoral and cellular immune responses, which opens up an avenue for the development of effective adjuvants against infectious diseases.
山药多糖（CYP）具有优异的佐剂活性，可以调节免疫反应，但低生物利用度限制了其临床应用。Pickering 乳液已被证明是一种有效的疫苗传递系统，可增强免疫反应。在这里，我们使用负载猪圆环病毒 2 的山药多糖 PLGA 稳定的 Pickering 乳液佐剂系统（CYP-PPAS）作为疫苗，并专注于研究其对小鼠体液和细胞免疫的佐剂活性。CYP-PPAS 提高了 PCV-2 抗原的加载效率，并表现出体外巨噬细胞的高抗原摄取效率。在体内，CYP-PPAS 比 CYP 或 PPAS 单独组更能促进引流淋巴结中的 DC 成熟。CYP-PPAS 还诱导了增殖指数和 CD4/CD8 比值的增加。同时，与 CYP 和 PPAS 组相比，CYP-PPAS 引起了更强的抗 PCV-2 IgG 和混合 Th1/Th2 免疫反应。具体而言，CYP-PPAS 组显示 CD107a、FasL 和 Granzyme B 分泌的高表达，以增强强烈的细胞毒性淋巴细胞反应。总体而言，CYP-PPAS 是一种促进体液和细胞免疫反应的成功佐剂系统，为开发针对传染病的有效佐剂开辟了道路。
