College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.
College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.
Colloids Surf B Biointerfaces. 2024 Dec;244:114144. doi: 10.1016/j.colsurfb.2024.114144. Epub 2024 Aug 6.
Traditional Alum adjuvants mainly elicit a Th2 humoral immune response, but fail to generate a robust Th1 cellular immune response. However, the cellular immune response is essential for vaccination against cancer and a number of chronic infectious diseases, including human immunodeficiency virus infection and tuberculosis. In our previous study, we demonstrated that the polysaccharide from Poria cocos (PCP) has the potential to serve as an immunologic stimulant, enhancing both humoral and cellular immune responses. However, this effect was only observed at high concentrations. In this study, to enhance the immune-stimulation effect of PCP and modify the type of immune response elicited by Alum adjuvant, we successfully developed a Pickering emulsion delivery system (PCP-Al-Pickering) using PCP-loaded Alhydrogel particles as the stabilizer. After optimization, the Pickering emulsion exhibited excellent storage capacity and effectively adsorbed the PCP and antigen. As an adjuvant delivery system, the PCP-Al-Pickering emulsion facilitated the antigen uptake by macrophages, increased the recruitment of cells at injection sites, improved the activation of dendritic cells in draining lymph nodes, elicited a potent and durable antibody response, and promoted the activation of CD4 and CD8 T cells. Importantly, the PCP-Al-Pickering emulsion adjuvant elicited a balanced Th1 and Th2 immune response, in comparison to Alum adjuvant. The PCP-Al-Pickering emulsion may serve as a safe and promising adjuvant delivery system to enhance immune responses.
传统的明矾佐剂主要引发 Th2 体液免疫反应，但不能产生强大的 Th1 细胞免疫反应。然而，细胞免疫反应对于预防癌症和一些慢性传染病（包括人类免疫缺陷病毒感染和结核病）至关重要。在我们之前的研究中，我们证明了从茯苓（PCP）中提取的多糖具有作为免疫刺激剂的潜力，可增强体液和细胞免疫反应。然而，这种效果仅在高浓度时观察到。在这项研究中，为了增强 PCP 的免疫刺激作用，并改变明矾佐剂引发的免疫反应类型，我们成功地使用负载 PCP 的 Alhydrogel 颗粒作为稳定剂开发了一种 Pickering 乳液递送系统（PCP-Al-Pickering）。经过优化，Pickering 乳液具有出色的储存能力，可有效吸附 PCP 和抗原。作为一种佐剂递送系统，PCP-Al-Pickering 乳液促进了巨噬细胞对抗原的摄取，增加了注射部位细胞的募集，改善了引流淋巴结中树突状细胞的激活，引发了强烈且持久的抗体反应，并促进了 CD4 和 CD8 T 细胞的激活。重要的是，与明矾佐剂相比，PCP-Al-Pickering 乳液佐剂引发了平衡的 Th1 和 Th2 免疫反应。PCP-Al-Pickering 乳液可能成为一种安全且有前途的佐剂递送系统，以增强免疫反应。
