Instituto de Patología Vegetal - Centro de Investigaciones Agropecuarias - Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), Camino 60 Cuadras Km 5,5 (X5020ICA), Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola, Camino 60 Cuadras Km 5,5 (X5020ICA), Córdoba, Argentina.
Gene. 2022 Nov 15;843:146806. doi: 10.1016/j.gene.2022.146806. Epub 2022 Aug 10.
Here, we report the identification and characterization of four novel DNA viruses from Welwitschia mirabilis transcriptomic and genomic datasets. Complete circular virus-like sequences with affinity to members of the Caulimoviridae and Geminiviridae families were detected and characterized from Welwitschia mirabilis genomic data. The two newly members of the Caulimoviridae family have been tentatively named as Welwitschia mirabilis virus 1 and 2 (WMV1-WMV2); whereas the two identified geminiviruses were named as Welwitschia mirabilis associated geminivirus A and B (WMaGVA-WMaGVB). Phylogenetic analysis suggests that WMV1-2 belong to a proposed genus of Caulimoviridae-infecting gymnosperms. WMaGVA-B are phylogenetically related with both mastreviruses and capulaviruses and likely represent a distinct evolutionary lineage within geminiviruses. Additionally, we detected several endogenous virus-like elements (EVE) linked to the discovered viruses in the recently reported W. mirabilis genome, suggesting a shared ancient evolutionary history of these viruses and the Welwithschia.
在这里，我们从百岁兰转录组和基因组数据集报告了四种新型 DNA 病毒的鉴定和特征。从百岁兰基因组数据中检测并鉴定了与 Caulimoviridae 和 Geminiviridae 家族成员具有亲和力的完整圆形病毒样序列。这两种新的 Caulimoviridae 家族成员被暂时命名为百岁兰病毒 1 和 2（WMV1-WMV2）；而鉴定出的两种双生病毒分别命名为百岁兰相关双生病毒 A 和 B（WMaGVA-WMaGVB）。系统发育分析表明，WMV1-2 属于一个拟议的 Caulimoviridae 感染裸子植物属。WMaGVA-B 与 mastreviruses 和 capulaviruses 具有系统发育关系，可能代表双生病毒中的一个独特进化谱系。此外，我们在最近报道的百岁兰基因组中检测到了与发现的病毒相关的几种内源性病毒样元件（EVE），表明这些病毒和 Welwithschia 具有共同的古老进化历史。
