Rashid Rubiya, Mohd Wani Sajad, Manzoor Shaziya, Masoodi F A, Masarat Dar Mohd
Department of Food Science and Technology, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Division of Food Science and Technology, Sher-e- Kashmir University of Agricultural Sciences and Technology-Kashmir, Srinagar, Jammu and Kashmir, India.
Food Chem. 2023 Jan 1;398:133871. doi: 10.1016/j.foodchem.2022.133871. Epub 2022 Aug 8.
Natural deep eutectic solvents (NADES) coupled with ultrasound assisted extraction (UAE) was evaluated as extraction techniques for bioactive compounds from apple pomace. Seven natural deep eutectic solvents (NADES) with different molar ratios were explored as green extraction solvents to evaluate extraction efficiency in terms of total flavonoid content (TFC), total phenolic content (TPC), lipid peroxidation inhibition (LBI) and antioxidant potential. The solvents which performed better than conventional solvent were DES1 (Choline chloride: glycerol (1:2)), DES2 (Choline chloride: lactic acid (1:3)) and DES5 ((Choline chloride: citric acid (1:1)). These solvents were further evaluated for optimisation of processing parameters in UAE-DES. The maximum TPC value was achieved using sonication time 40 min, water 30 %, solid/liquid ratio 1:30, temperature 40 °C, acoustic intensity 83.2 W/cm and duty cycle 75 %. HPLC of optimised extract was utilised for quantification of polyphenol compounds showing quercetin as main compound followed by chlorogenic acid, gallic acid, phloretin, phloridizin and rutin.
评估了天然深共熔溶剂（NADES）与超声辅助萃取（UAE）相结合作为从苹果渣中提取生物活性化合物的技术。研究了七种不同摩尔比的天然深共熔溶剂（NADES）作为绿色萃取溶剂，以总黄酮含量（TFC）、总酚含量（TPC）、脂质过氧化抑制（LBI）和抗氧化潜力来评估萃取效率。表现优于传统溶剂的溶剂为DES1（氯化胆碱：甘油（1:2））、DES2（氯化胆碱：乳酸（1:3））和DES5（氯化胆碱：柠檬酸（1:1））。对这些溶剂进一步评估以优化UAE-DES中的工艺参数。使用超声处理时间40分钟、水30%、固液比1:30、温度40°C、声强83.2W/cm和占空比75%时可获得最大TPC值。对优化提取物进行高效液相色谱分析以定量多酚化合物，结果表明槲皮素是主要化合物，其次是绿原酸、没食子酸、根皮素、根皮苷和芦丁。
