Konikoff F, Shoenfeld Y, Isenberg D A, Theodor F, Moroz C
Hepatogastroenterology. 1987 Apr;34(2):65-7.
Serum ferritin levels in chronic liver diseases were measured by an enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies (McAb) against placental ferritin. One of the McAbs (CM-H9) recognizes a specific placental-like isoferritin (PLF) only, while the other McAb (CM-G8) recognizes an isoform (CF) common to human placenta, liver and spleen. The different diseases studied were primary biliary cirrhosis (PBC-14 patients), chronic active hepatitis (CAH-12 patients), alcoholic cirrhosis (AC-18 patients) and cryptogenic cirrhosis (CRY-26 patients). Increased levels of both isoferritine were found in all these liver disorders, with an overall incidence of 80% for CF and 40% for PLF. The mean level of CF was significantly above normal in PBC, AC and CRY and that of PLF in PBC and CRY. Circulating placental-type isoferritins have not been previously described in patients with liver disorders. Our findings indicate that elevation of serum ferritin in liver diseases is caused by different isoferritin components.
采用两种抗胎盘铁蛋白的单克隆抗体，通过酶联免疫吸附测定法（ELISA）检测慢性肝病患者的血清铁蛋白水平。其中一种单克隆抗体（CM-H9）仅识别一种特定的胎盘样异铁蛋白（PLF），而另一种单克隆抗体（CM-G8）识别一种人类胎盘、肝脏和脾脏共有的异构体（CF）。所研究的不同疾病包括原发性胆汁性肝硬化（PBC，14例患者）、慢性活动性肝炎（CAH，12例患者）、酒精性肝硬化（AC，18例患者）和隐源性肝硬化（CRY，26例患者）。在所有这些肝脏疾病中均发现两种异铁蛋白水平升高，CF的总体发生率为80%，PLF为40%。CF的平均水平在PBC、AC和CRY中显著高于正常，PLF的平均水平在PBC和CRY中显著高于正常。此前尚未在肝病患者中描述过循环胎盘型异铁蛋白。我们的研究结果表明，肝病患者血清铁蛋白升高是由不同的异铁蛋白成分引起的。
