Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
J Endod. 2022 Nov;48(11):1400-1406. doi: 10.1016/j.joen.2022.08.003. Epub 2022 Aug 12.
The aim of this study was to evaluate osteoclastogenesis and dental resorption resulting from endodontic infection in wild-type (WT) and tumor necrosis factor receptor 1 genetically deficient (TNFR1 KO) mice.
After approval by the ethics committee on the use of animals, 40 mice were distributed into 2 experimental groups based on time periods: 14 days (n = 10 WT mice and n = 10 TNFR1 KO mice) and 42 days (n = 10 WT mice and n = 10 TNFR1 KO mice). After these periods, morphometric analysis was performed using bright field and fluorescence microscopy and tartrate-resistant acid phosphatase histoenzymology to identify osteoclasts. One-way analysis of variance followed by the Tukey post hoc test was used for the statistical analysis (α = 0.05).
WT mice in the 42-day period had a greater apical dental resorption in the distal root of the first molar than TNFR1 KO mice (P < .05). On the other hand, TNFR1 KO mice showed a smaller number of osteoclasts on the dental surface than WT mice (P < .05).
WT mice with apical periodontitis had more extensive apical dental resorptions and a larger number of osteoclasts on the tooth surface than TNFR1 KO mice.
本研究旨在评估WT 型（野生型）和 TNF 受体 1 基因缺陷型（TNFR1 KO）小鼠的牙髓感染导致的破骨细胞形成和牙吸收。
在动物使用伦理委员会批准后，将 40 只小鼠根据时间分为 2 个实验组：14 天（n=10 只 WT 小鼠和 n=10 只 TNFR1 KO 小鼠）和 42 天（n=10 只 WT 小鼠和 n=10 只 TNFR1 KO 小鼠）。在此期间结束后，使用明场和荧光显微镜以及抗酒石酸酸性磷酸酶组织化学法进行形态计量分析，以识别破骨细胞。采用单因素方差分析和 Tukey 事后检验进行统计学分析（α=0.05）。
42 天组的 WT 小鼠第一磨牙远中根尖的牙吸收比 TNFR1 KO 小鼠更广泛（P<0.05）。另一方面，TNFR1 KO 小鼠的牙表面破骨细胞数量比 WT 小鼠少（P<0.05）。
WT 型伴根尖周炎的小鼠的根尖牙吸收比 TNFR1 KO 小鼠更广泛，牙表面的破骨细胞数量也更多。
