Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Brazil.
Scanning. 2023 Apr 17;2023:4619503. doi: 10.1155/2023/4619503. eCollection 2023.
The aim of this study was to evaluate the sensitivity, specificity, and predictive values of the fluorescence microscopy method in the detection of apical dental reabsorption after induction of apical periodontitis in animal models. Forty-first molars of mice, aged 6 to 8 weeks, had their root canals exposed to the oral environment or were maintained healthy as controls ( = 20). After 14 and 42 days, mice were euthanized and tissues were collected for histological evaluation by means of bright field and fluorescence microscopy. The accuracy of fluorescence microscopy in identifying apical external dental resorption was investigated using a diagnostic validation test based on the sensitivity (S) and specificity (E) properties. Bright-field microscopy revealed a higher number of specimens with scores of 1 to 3 - absence of apical dental resorption ( = 29; 52%), while fluorescence microscopy revealed a higher number of specimens with scores of 4 to 6 - presence of apical dental resorption ( = 37; 66%). Out of 56 specimens, 26 were TP, 11 were FP, and 19 were TN. No FN result was observed. Fluorescence microscopy presented a sensitivity value of 1, similar to the bright-field method, while specificity was lower (0.633). The accuracy of the fluorescent method to detect apical dental resorption was 0.804. Fluorescence microscopy revealed a higher number of false positive apical dental resorption than bright-field microscopy. The detection of apical dental resorption was not impacted by the sensitivity of the method but by its specificity.
本研究旨在评估荧光显微镜法在检测动物模型中诱导根尖周炎后根尖牙吸收的敏感性、特异性和预测值。6 至 8 周龄的 41 颗磨牙，将其根管暴露于口腔环境或作为对照保持健康（n=20）。14 和 42 天后，处死小鼠并收集组织，通过明场和荧光显微镜进行组织学评估。使用基于灵敏度（S）和特异性（E）特性的诊断验证试验，研究了荧光显微镜在识别根尖外部牙吸收中的准确性。明场显微镜显示，有较多评分在 1 至 3 级（无根尖牙吸收）的标本（n=29；52%），而荧光显微镜显示，有较多评分在 4 至 6 级（存在根尖牙吸收）的标本（n=37；66%）。在 56 个标本中，26 个为 TP，11 个为 FP，19 个为 TN。未观察到 FN 结果。荧光显微镜的敏感性值为 1，与明场法相似，而特异性较低（0.633）。荧光法检测根尖牙吸收的准确性为 0.804。荧光显微镜显示的假阳性根尖牙吸收比明场显微镜多。根尖牙吸收的检测不受方法敏感性的影响，而是受其特异性的影响。
