Oppedal B R, Brandtzaeg P, Kemshead J T
Histopathology. 1987 Apr;11(4):351-62. doi: 10.1111/j.1365-2559.1987.tb02640.x.
Tissue specimens of neural crest-derived structures like normal spinal and sympathetic ganglia and adrenals of adults, infants and fetuses were used as immunohistochemical test substrates for a panel of 10 monoclonal antibodies to neuroblastoma cells raised by immunization with a variety of immunogens (fetal brain, neuroblastoma cell lineages, chick retinal cells or purified Thy-1 antigen). Antigen expression varied among normal structures such as ganglion cells, satellite cells, various nerve fibres, and different cells in the adrenal cortex. This variability in immunoreactivity shown by different cell types and structures was comparable to that seen when the same panel of monoclonal antibodies was applied to neuroblastomas and to ganglioneuroblastomas of different degrees of maturation and differentiation. The neoplasms thus seemed to reflect the normal maturation in the neuroectodermally-derived structures. Also, the studies give indications as to the cell of origin of the undifferentiated neuroblastomas.
取自成人、婴儿和胎儿的正常脊髓、交感神经节和肾上腺等神经嵴衍生结构的组织标本，被用作免疫组织化学检测底物，用于检测一组10种针对神经母细胞瘤细胞的单克隆抗体。这些单克隆抗体是通过用多种免疫原（胎儿脑、神经母细胞瘤细胞系、鸡视网膜细胞或纯化的Thy-1抗原）免疫制备的。抗原表达在正常结构之间存在差异，如神经节细胞、卫星细胞、各种神经纤维以及肾上腺皮质中的不同细胞。不同细胞类型和结构所显示的这种免疫反应性差异，与将同一组单克隆抗体应用于不同成熟度和分化程度的神经母细胞瘤和神经节神经母细胞瘤时所观察到的情况相当。因此，这些肿瘤似乎反映了神经外胚层衍生结构中的正常成熟过程。此外，这些研究还为未分化神经母细胞瘤的起源细胞提供了线索。
