Departamento de Psicobiología, Facultad Psicología Universidad Nacional de Educación a Distancia (UNED), C/Juan del Rosal 10, 28040 Madrid, Spain; Instituto Mixto de Investigación Escuela Nacional de Sanidad-UNED (IMIENS), Spain.
Departamento de Psicobiología, Facultad Psicología Universidad Nacional de Educación a Distancia (UNED), C/Juan del Rosal 10, 28040 Madrid, Spain.
Behav Brain Res. 2023 Jan 5;436:114055. doi: 10.1016/j.bbr.2022.114055. Epub 2022 Aug 11.
The objective of this study was to investigate the orexin and POMC populations in the hypothalamic nuclei of male Wistar rats after the activity-based anorexia (ABA) procedure. Four groups were established based on food restriction and activity: activity (A), ABA, diet (D) and control (C). The ABA protocol consisted of free access to a running wheel for a period of 22 h and access to food for 1 h. When the animals in the ABA group reached the ABA criterion, were sacrificed, and their brains were collected and serially sectioned. The free-floating sections were processed for orexin and POMC immunostaining. The number of orexin A-ir cells in the perifornical-dorsomedial-hypothalamus continuum (PFD) and lateral hypothalamus (LH) and the number of POMC-ir cells in the arcuate nucleus (Arc) were estimated. Data on food intake, body weight and wheel turns were also analyzed. The ABA procedure caused a significant decrease in body weight along with a significant increase in activity. Moreover, at the end of the ABA procedure, the number of POMC-ir cells decreased in the Arc in the A group, and significantly more in the ABA group, and the number of orexin A-ir positive cells decreased in the LH in D and ABA groups. The differential decrease in POMC in the ABA group emphasizes the importance of the melanocortin system in the maintenance of ABA, but more research is needed to elucidate the involvement of this peptide in the mechanism that promotes and maintains anorexia nervosa and how increased activity may interact with all these processes.
本研究旨在探讨活动限制型厌食症（ABA）程序后雄性 Wistar 大鼠下丘脑核内的食欲素和 POMC 群体。根据食物限制和活动情况建立了四个组：活动（A）、ABA、饮食（D）和对照（C）。ABA 方案包括自由使用跑步轮 22 小时，以及 1 小时的食物摄取时间。当 ABA 组的动物达到 ABA 标准时，将其处死，并收集其大脑进行连续切片。进行游离漂浮切片，以进行食欲素和 POMC 免疫染色。估计了 PFD 和 LH 中的食欲素 A-ir 细胞数量以及 Arc 中的 POMC-ir 细胞数量。还分析了食物摄入量、体重和车轮转动的数据。ABA 程序导致体重显著下降，同时活动显著增加。此外，在 ABA 程序结束时，A 组的 Arc 中 POMC-ir 细胞数量减少，ABA 组的数量显著减少，D 和 ABA 组的 LH 中食欲素 A-ir 阳性细胞数量减少。ABA 组 POMC 的差异减少强调了黑素皮质素系统在维持 ABA 中的重要性，但需要进一步研究来阐明该肽在促进和维持神经性厌食症的机制中的作用，以及活动增加如何与所有这些过程相互作用。
