Shandong Laboratory of Yantai Advanced Materials and Green Manufacturing, Yantai 264006, China; Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering, Yantai 264010, PR China.
Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering, Yantai 264010, PR China; CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics (LICP), Chinese Academy of Sciences (CAS), Lanzhou 730000, PR China.
Fitoterapia. 2022 Oct;162:105280. doi: 10.1016/j.fitote.2022.105280. Epub 2022 Aug 12.
Three unreported dammarane-type triterpenoids with rare skeletons (1-3), along with one undescribed gypenoside (4), were isolated from the aerial parts of Gynostemma pentaphyllum using diverse chromatographic materials and pre-HPLC. Their structures were elucidated on the basis of spectroscopic and spectrometric data, while the absolute configurations of 1-3 were assessed via electronic circular dichroism (ECD) analyses. Notably, compounds 1-3 possess a 3,19-hemiketal bridge in the A ring. Saponin 4 possesses an unreported 20,25-oxa structural moiety. Their antiproliferative effects against HepG2, MCF-7, and DU145 cell lines were screened. Compounds 1-3 displayed moderate cytotoxicity with IC values ranging from 13.7 ± 0.2 to 32.0 ± 1.7 μM.
从绞股蓝的地上部分用不同的色谱材料和预 HPLC 分离得到三个未报道的达玛烷型三萜（1-3），以及一个未描述的绞股蓝皂苷（4）。基于光谱和光谱数据阐明了它们的结构，而通过电子圆二色谱（ECD）分析评估了 1-3 的绝对构型。值得注意的是，化合物 1-3 在 A 环中具有 3,19-半缩酮桥。皂苷 4 具有未报道的 20,25-噁结构部分。筛选了它们对 HepG2、MCF-7 和 DU145 细胞系的抗增殖作用。化合物 1-3 表现出中等的细胞毒性，IC 值范围为 13.7 ± 0.2 至 32.0 ± 1.7 μM。
