Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, P. R. China.
State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Centre for New Organic Matter, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Centre for Analytical Sciences, College of Chemistry and School of Medicine, Nankai University, Tianjin, 300071, P. R. China.
Angew Chem Int Ed Engl. 2022 Sep 26;61(39):e202210285. doi: 10.1002/anie.202210285. Epub 2022 Aug 25.
Viral encephalitis is an inflammatory disease of the brain parenchyma and caused by various viral infections. In vivo monitoring of the progression of viral infections can aid accurate diagnosis of viral encephalitis and effective intervention. We developed an activatable and reversible virus-mimicking near-infrared II nanoprobe consisting of an Fe -coordinated, viral protein-decorated vesicle encapsulating PbS quantum dots with a 1300 nm fluorescence emission. The probe can cross the blood-brain barrier and monitor real-time changes in reactive oxygen and nitrogen species concentrations during viral infection by tuning the quenching level of quantum dots and regulating the fusion-fission behavior of vesicles via changes in Fe oxidation state. This switching strategy reduces background noise and improves detection sensitivity, making this nanoprobe a promising imaging agent for dynamic visualization of viral encephalitis and future clinical applications.
病毒性脑炎是一种脑实质炎症性疾病，由各种病毒感染引起。对病毒感染进展的体内监测有助于病毒性脑炎的准确诊断和有效干预。我们开发了一种可激活和可还原的类似病毒的近红外二区纳米探针，该探针由 Fe 配位的、病毒蛋白修饰的囊泡包裹 PbS 量子点组成，具有 1300nm 的荧光发射。该探针可以穿过血脑屏障，并通过调节量子点的猝灭水平和通过 Fe 氧化态的变化调节囊泡的融合-裂变行为，来监测病毒感染过程中活性氧和氮物种浓度的实时变化。这种开关策略降低了背景噪声，提高了检测灵敏度，使这种纳米探针成为动态可视化病毒性脑炎和未来临床应用的有前途的成像剂。
