Habibzadeh Sareh, Eskandarion Solmauz, Marashi Seyed Mahmoud Amin, Yunesi Ghazal, Kharazifard Mohamadjavad
Department of Prosthodontics, School of Dentistry, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
Front Dent. 2021 Apr 5;18:8. doi: 10.18502/fid.v18i8.6101. eCollection 2021.
: This study aimed to evaluate the in vitro antifungal efficacy of addition of silver nanoparticles (SNPs) to Mucopren® silicone soft liner material. : Twenty disc samples (8 × 2 mm) of Mucopren® silicone soft liner containing 0wt% (control), 0.5wt%, 1wt%, 2wt%, and 3wt% SNPs were fabricated. Samples were powdered and added to 150 mL of Sabouraud dextrose agar culture medium and placed on separate culture dish plates. Each plate was inoculated with 10 colony forming units per milliliter (CFUs/mL) of Candida albicans (PTCC 5027) according to the CLSI protocol, and incubated at 37℃. The colony count was verified at 24 h, and the antifungal effect of the samples was evaluated according to the percentage of viable cells in the 2 subgroups with/without thermocycling. Data were analyzed using SPSS version 20 via ANOVA and t-test (P<0.05). All experimental groups showed higher antifungal activity than the control group, and this effect was dose-dependent (P<0.05). The lowest colony count was recorded in the 3wt% group. Thermocycling had no significant effect on the antifungal efficacy, except in 0.5wt% concentration of SNPs (P=0.013). Addition of SNPs to Mucopren soft liner conferred antifungal effects. Further mechanical stability and toxicity studies are still required.
本研究旨在评估向Mucopren®硅酮软衬材料中添加银纳米颗粒（SNP）的体外抗真菌效果。制备了20个直径8×2mm的Mucopren®硅酮软衬圆盘样品，分别含0wt%（对照）、0.5wt%、1wt%、2wt%和3wt%的SNP。将样品磨成粉末，加入150mL沙氏葡萄糖琼脂培养基中，并置于单独的培养皿中。根据CLSI方案，每个平板接种每毫升10个白色念珠菌（PTCC 5027）菌落形成单位（CFUs/mL），并在37℃下培养。在24小时时检查菌落数，并根据有无热循环的两个亚组中活细胞的百分比评估样品的抗真菌效果。使用SPSS 20版通过方差分析和t检验（P<0.05）对数据进行分析。所有实验组均显示出比对照组更高的抗真菌活性，且这种效果呈剂量依赖性（P<0.05）。3wt%组的菌落数最低。除了0.5wt%浓度的SNP外，热循环对抗真菌效果没有显著影响（P=0.013）。向Mucopren软衬中添加SNP具有抗真菌作用。仍需要进一步进行机械稳定性和毒性研究。
