Stanker L H, Wyrobek A, Balhorn R
Hybridoma. 1987 Jun;6(3):293-303. doi: 10.1089/hyb.1987.6.293.
Nine monoclonal antibodies to human protamine, hup1a, 1b, 1c, 1d, 1e, 2a, 2b, A, and B, have been isolated and partially characterized. Enzyme-linked immunoabsorption assay analyses with HPLC-separated human protamine 1 and protamine 2+3 mixture identified five of these antibodies as specific for human protamine 1, two antibodies specific for protamine 2+3 mixture and two monoclonal antibodies reactive with all three human protamines. These findings were confirmed by immunoblotting. None of the antibodies reacted with poly-arginine or somatic histone proteins. Additional analyses with bull, boar, and ram protamines indicated that all of the monoclonal antibodies except hupA are specific for human protamine. HupA reacted with protamines from all of the species tested. These studies suggest that each of the antibodies recognizes one of at least four distinct epitopes on protamine.
已分离出九种针对人精蛋白的单克隆抗体，即hup1a、1b、1c、1d、1e、2a、2b、A和B，并对其进行了部分特性鉴定。采用HPLC分离的人精蛋白1和精蛋白2 + 3混合物进行酶联免疫吸附分析，确定其中五种抗体对人精蛋白1具有特异性，两种抗体对精蛋白2 + 3混合物具有特异性，还有两种单克隆抗体可与所有三种人精蛋白发生反应。这些结果通过免疫印迹得到了证实。没有一种抗体与聚精氨酸或体细胞组蛋白发生反应。对公牛、公猪和公羊精蛋白的进一步分析表明，除hupA外，所有单克隆抗体都对人精蛋白具有特异性。hupA与所有测试物种的精蛋白都发生反应。这些研究表明，每种抗体识别精蛋白上至少四个不同表位中的一个。
