Rodriguez-Martinez H, Courtens J L, Kvist U, Plöen L
Department of Anatomy and Histology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
J Reprod Fertil. 1990 Jul;89(2):591-5. doi: 10.1530/jrf.0.0890591.
Protamine was specifically demonstrated in boar spermatozoa collected from the rete testis, caput, corpus and cauda epididymidis and the ejaculate by immunoelectron microscopy, using anti-boar or anti-ram protamine antisera and an indirect post-embedding immunogold technique. Spermatozoa from all collection sites stained after incubation although with different degrees of labelling. Controls were negative. Labelling increased from the rete testis towards the epididymal corpus, where it was most intense, decreasing sharply thereafter. The weakest binding of the assayed antibodies was obtained in the ejaculated spermatozoa but it could be reversed by in-vitro induction of chromatin decondensation with sodium dodecyl sulphate and the metal-chelating EDTA. The finding of a significant decrease in the immunolabelling detected from the corpus epididymidis onwards indicates a critical point for the interaction between DNA and the protamines in boar spermatozoa during the epididymal maturation.
利用抗猪或抗公羊鱼精蛋白抗血清以及间接包埋后免疫金技术，通过免疫电子显微镜在从睾丸网、附睾头、附睾体、附睾尾采集的公猪精子以及射精精液中特异性地证实了鱼精蛋白的存在。尽管标记程度不同，但来自所有采集部位的精子在孵育后均被染色。对照为阴性。标记从睾丸网向附睾体增加，在附睾体处最为强烈，此后急剧下降。在所检测的抗体中，射精精子的结合最弱，但用十二烷基硫酸钠和金属螯合剂乙二胺四乙酸进行体外染色质解聚诱导可使其逆转。从附睾体开始检测到的免疫标记显著减少，这一发现表明在附睾成熟过程中，公猪精子中DNA与鱼精蛋白之间相互作用存在一个关键点。
