克罗地亚甲状腺功能检测的实验室政策和实践：克罗地亚医学生物化学和实验室医学学会实验室内分泌学工作组调查。

Laboratory policies and practices for thyroid function tests in Croatia: survey on behalf of Working Group for Laboratory Endocrinology of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

机构信息

Laboratory of Endocrinology, Department of Oncology and Nuclear Medicine, Sestre Milosrdnice University Hospital Center, Zagreb, Croatia.

University Department of Chemistry, Sestre Milosrdnice University Hospital Center, Zagreb, Croatia.

出版信息

Biochem Med (Zagreb). 2022 Oct 1;32(3):030702. doi: 10.11613/BM.2022.030702. Epub 2022 Aug 5.

DOI:10.11613/BM.2022.030702

PMID:35966262

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9344866/

Abstract

INTRODUCTION

Laboratory plays important part in screening, diagnosis, and management of thyroid disorders. The aim of this study was to estimate current laboratory preanalytical, analytical and postanalytical practices and policies in Croatia.

MATERIALS AND METHODS

Working Group for Laboratory Endocrinology of the Croatian Society of Medical Biochemistry and Laboratory Medicine designed a questionnaire with 27 questions and statements regarding practices and protocols in measuring thyroid function tests. The survey was sent to 111 medical biochemistry laboratories participating in external quality assurance scheme for thyroid hormones organized by Croatian Centre for Quality Assessment in Laboratory Medicine. Data is presented as absolute numbers and proportions.

RESULTS

Fifty-three participants returned the questionnaire. Response rate varied depending on question, yielding a total survey response rate of 46-48%. All respondents perform thyroid stimulating hormone (TSH). From all other thyroid tests, most performed is free thyroxine (37/53) and least TSH-stimulating immunoglobulin (1/53). Laboratories are using nine different immunoassay methods. One tenth of laboratories is verifying manufacturer's declared limit of quantification for TSH and one third is verifying implemented reference intervals for all performed tests. Most of laboratories (91%) adopt the manufacturer's reference interval for adult population. Reference intervals for TSH are reported with different percentiles (90, 95 or 99 percentiles).

CONCLUSION

This survey showed current practices and policies in Croatian laboratories regarding thyroid testing. The results identified some critical spots and will serve as a foundation in creating national guidelines in order to harmonize laboratory procedures in thyroid testing in Croatia.

摘要

简介

实验室在甲状腺疾病的筛查、诊断和治疗中起着重要作用。本研究旨在评估克罗地亚当前的实验室分析前、分析中和分析后实践和政策。

材料和方法

克罗地亚医学生物化学和实验室医学学会的实验室内分泌学工作组设计了一份包含 27 个问题和陈述的问卷，涉及甲状腺功能检测的实践和方案。该调查被发送给了 111 家参加由克罗地亚实验室医学质量评估中心组织的甲状腺激素外部质量保证计划的医学生化实验室。数据以绝对值和比例表示。

结果

53 名参与者回复了问卷。回复率因问题而异，总调查回复率为 46-48%。所有受访者均进行促甲状腺激素（TSH）检测。在所有其他甲状腺检测中，最常进行的是游离甲状腺素（37/53），而 TSH 刺激免疫球蛋白（1/53）最少。实验室使用了九种不同的免疫测定方法。十分之一的实验室正在验证 TSH 的制造商声明的定量下限，三分之一的实验室正在验证所有已执行检测的实施参考区间。大多数实验室（91%）采用制造商为成人人群设定的参考区间。TSH 的参考区间报告采用不同的百分位数（90%、95%或 99%）。