School of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang, Henan, China.
PeerJ. 2022 Aug 9;10:e13786. doi: 10.7717/peerj.13786. eCollection 2022.
The basic helix loop helix (bHLH) transcription factor perform essential roles in plant development and abiotic stress. Here, a total of 122 bHLH family members were identified from the physic nut ( L.) genomic database. Chromosomal localization results showed that 120 members were located on 11 chromosomes. The phylogenetic tree manifested that the could be grouped into 28 subfamilies. Syntenic analysis showed that there were 10 bHLH collinear genes among the physic nut, and . These genes, except , were highly expressed in various tissues of the physic nut, implying a key role in plant development. Gene expression profiles showed that ten genes (especially , and ) correspond to both salinity and drought stresses; while eight genes only respond to salinity and another eight genes only respond to drought stress. Moreover, the protein interaction network revealed that the are involved in growth, development and stress signal transduction pathways. These discoveries will help to excavate several key genes may involve in salt or drought stresses and seed development, elucidate the complex transcriptional regulation mechanism of genes and provide the theoretical basis for stress response and genetic improvement of physic nut.
基本螺旋-环-螺旋 (bHLH) 转录因子在植物发育和非生物胁迫中发挥着重要作用。在这里，从麻疯树基因组数据库中鉴定出了总共 122 个 bHLH 家族成员。染色体定位结果表明，120 个成员位于 11 条染色体上。系统发育树表明， 可以分为 28 个亚家族。共线性分析表明，在麻疯树、 和 之间存在 10 个 bHLH 共线性基因。这些基因除 外，在麻疯树的各种组织中均高度表达，表明它们在植物发育中起关键作用。基因表达谱显示，十个基因（特别是 、 和 ）对应盐胁迫和干旱胁迫；而八个基因仅对盐胁迫有反应，另外八个基因仅对干旱胁迫有反应。此外，蛋白质相互作用网络表明， bHLH 基因参与生长、发育和胁迫信号转导途径。这些发现将有助于挖掘出一些可能参与盐或干旱胁迫和种子发育的关键基因，阐明 基因的复杂转录调控机制，为麻疯树的胁迫响应和遗传改良提供理论基础。
