Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, PR China.
Gene. 2013 Jul 25;524(2):124-32. doi: 10.1016/j.gene.2013.04.047. Epub 2013 May 3.
The WRKY proteins, which contain highly conserved WRKYGQK amino acid sequences and zinc-finger-like motifs, constitute a large family of transcription factors in plants. They participate in diverse physiological and developmental processes. WRKY genes have been identified and characterized in a number of plant species. We identified a total of 58 WRKY genes (JcWRKY) in the genome of the physic nut (Jatropha curcas L.). On the basis of their conserved WRKY domain sequences, all of the JcWRKY proteins could be assigned to one of the previously defined groups, I-III. Phylogenetic analysis of JcWRKY genes with Arabidopsis and rice WRKY genes, and separately with castor bean WRKY genes, revealed no evidence of recent gene duplication in JcWRKY gene family. Analysis of transcript abundance of JcWRKY gene products were tested in different tissues under normal growth condition. In addition, 47 WRKY genes responded to at least one abiotic stress (drought, salinity, phosphate starvation and nitrogen starvation) in individual tissues (leaf, root and/or shoot cortex). Our study provides a useful reference data set as the basis for cloning and functional analysis of physic nut WRKY genes.
WRKY 蛋白包含高度保守的 WRKYGQK 氨基酸序列和锌指样基序，是植物中一类重要的转录因子家族。它们参与多种生理和发育过程。已经在许多植物物种中鉴定和描述了 WRKY 基因。我们在麻疯树（Jatropha curcas L.）基因组中总共鉴定了 58 个 WRKY 基因（JcWRKY）。根据它们保守的 WRKY 结构域序列，所有的 JcWRKY 蛋白都可以被分配到之前定义的 I-III 组之一。与拟南芥和水稻 WRKY 基因以及蓖麻 WRKY 基因的系统发育分析表明，JcWRKY 基因家族没有最近基因复制的证据。在正常生长条件下，分析了不同组织中 JcWRKY 基因产物的转录丰度。此外，47 个 WRKY 基因在单个组织（叶、根和/或茎皮层）中至少对一种非生物胁迫（干旱、盐胁迫、磷饥饿和氮饥饿）有反应。我们的研究提供了一个有用的参考数据集，为克隆和功能分析麻疯树 WRKY 基因奠定了基础。
