Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
Department of Clinical Science and Education, Södersjukhuset, Karolinska Institutet, Stockholm, Sweden.
J Diabetes Res. 2022 Aug 3;2022:3975147. doi: 10.1155/2022/3975147. eCollection 2022.
Expression of the transient receptor potential (TRP) channel genes and their isoforms in the alpha-cells and the beta-cells of the human islets of Langerhans has not been studied in detail. In this study, we have analyzed the RNA sequencing data obtained from purified human alpha-cells and beta-cells to identify the genes and their isoforms that are expressed differentially in these two cell types. We found that , , , , and were differentially expressed in these two cell types. , , and were expressed at a higher level in the beta-cells than in the alpha-cells. and were expressed at a higher level in the alpha-cells than in the beta-cells. The isoform was expressed at a 45-fold higher level in the alpha-cells compared to the beta-cells. Expression of was 200-fold and was 25-fold higher in the beta-cells than in the alpha-cells. Our study has demonstrated the relative abundance of expression of the TRP channel genes and their isoforms in the human alpha-cells and the beta-cells.
人胰岛α细胞和β细胞中转瞬受体电位（TRP）通道基因及其亚型的表达尚未详细研究。在这项研究中，我们分析了从纯化的人α细胞和β细胞中获得的 RNA 测序数据，以鉴定这两种细胞类型中差异表达的基因及其亚型。我们发现 、 、 、 、 在这两种细胞类型中差异表达。 、 、 和 在β细胞中的表达水平高于α细胞。 和 在α细胞中的表达水平高于β细胞。 亚型在α细胞中的表达水平比β细胞高 45 倍。 在β细胞中的表达水平比α细胞高 200 倍， 在β细胞中的表达水平比α细胞高 25 倍。我们的研究表明了 TRP 通道基因及其亚型在人胰岛α细胞和β细胞中的相对表达丰度。
