Deng Shiming, Xiao Qiang, Xu Cigui, Hong Jian, Deng Zhijun, Jiang Dan, Luo Shijia
Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Hubei Minzu University, Enshi 445000, Hubei Province, China.
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Plant Divers. 2021 Dec 30;44(4):417-427. doi: 10.1016/j.pld.2021.12.001. eCollection 2022 Jul.
Dove tree (), a tertiary vestige species, is well-adapted to cool conditions. Dormancy in seed lasts for an extremely long period of time, typically between 3 and 4 years, and this characteristic makes the species an excellent model for studying the mechanisms of seed dormancy. The molecular mechanisms governing germination control in are still unknown. Seed stratification have been reported to enhance germination in recalcitrant seeds. We performed a widely targeted metabolome profiling to identify metabolites and associated pathways in seeds from six different moist sand stratification durations (0-30 months) using the ultra-high-performance liquid chromatography-Q Exactive Orbitrap-Mass spectrometry. There was an increasing germination rate with prolonged stratification durations (12-30 months). Furthermore, we detected 10,008 metabolites in the stratified seeds. We also detected 48 differentially accumulated metabolites (DAMs) between all stratification periods in the seeds, with 10 highly conserved metabolites. Most of the differentially accumulated metabolites between unstratified and stratified seeds were enriched in purine metabolism, pyrimidine metabolism, flavone and flavonol biosynthesis, phenylpropanoid biosynthesis, and arginine biosynthesis pathways. Key phytohormones, abscisic acid, indole-3 acetic acid, and sinapic acid were differentially accumulated in the seeds and are predicted to regulate dormancy in . We have provided extensive metabolic information useful for future works on dove tree germination study.
珙桐是一种第三纪残遗物种，非常适应凉爽的环境。其种子休眠期极长，通常为3至4年，这一特性使该物种成为研究种子休眠机制的理想模型。目前，关于珙桐种子萌发控制的分子机制尚不清楚。据报道，种子层积处理可提高顽拗性种子的萌发率。我们利用超高效液相色谱- Q Exactive Orbitrap质谱联用技术，对来自六个不同湿沙层积处理时长（0至30个月）的珙桐种子进行了广泛靶向代谢组分析，以鉴定代谢产物及相关途径。随着层积处理时长的延长（12至30个月），种子的萌发率不断提高。此外，我们在层积处理的种子中检测到了10,008种代谢产物。我们还在种子的所有层积处理时期之间检测到48种差异积累代谢产物（DAMs），其中有10种高度保守的代谢产物。未层积和层积种子之间的大多数差异积累代谢产物在嘌呤代谢、嘧啶代谢、黄酮和黄酮醇生物合成、苯丙烷生物合成以及精氨酸生物合成途径中富集。关键植物激素脱落酸、吲哚- 3 -乙酸和芥子酸在种子中差异积累，并预计可调节珙桐种子的休眠。我们提供了丰富的代谢信息，有助于未来开展珙桐种子萌发研究。
