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通过全基因组重测序对竹种间遗传变异及其与拉伸强度的关联进行研究。

Study of genetic variation and its association with tensile strength among bamboo species through whole genome resequencing.

作者信息

Del Giudice Lorenzo, Bazakos Christos, Vassiliou Michalis F

机构信息

Chair of Seismic Design and Analysis, Institute of Structural Engineering, ETH Zurich, Zurich, Switzerland.

Institute of Plant Breeding and Genetic Resources, ELGO-Dimitra, Thessaloniki, Greece.

出版信息

Front Plant Sci. 2022 Jul 27;13:935751. doi: 10.3389/fpls.2022.935751. eCollection 2022.

Abstract

Moso bamboo () is a versatile plant species that is widely used as a construction material by many low-income countries due to the lack of major construction materials such as steel and reinforced concrete. It is also widely used in China. Bamboo is an economically sustainable material that behaves exceptionally in natural disasters such as earthquakes and it can offer viable solutions for contemporary engineering challenges. Despite bamboo's potential in the engineering sector, biological features such as its long generation time, its large genome size, and its polyploidy are constraining factors for genetic and genomic studies that potentially can assist the breeding efforts. This study re-sequenced 8 species and 18 natural accessions of , generating a large set of functionally annotated molecular markers (Single Nucleotide Polymorphisms (SNPs) and InDels) providing key genomic resource information. Moreover, all this genomic information was used to carry out a preliminary genome-wide association analysis and several candidate genes were identified to be correlated with a mechanical property that is of high interest to structural engineers: its tensile strength normal to its fibers (i.e., splitting).

摘要

毛竹()是一种用途广泛的植物物种,由于许多低收入国家缺乏钢铁和钢筋混凝土等主要建筑材料,它被广泛用作建筑材料。在中国它也被广泛使用。竹子是一种经济上可持续的材料,在地震等自然灾害中表现出色,并且可以为当代工程挑战提供可行的解决方案。尽管竹子在工程领域具有潜力,但其较长的世代时间、较大的基因组大小和多倍体等生物学特性是遗传和基因组研究的制约因素,而这些研究可能有助于育种工作。本研究对8个物种和18个毛竹自然种质进行了重测序,生成了大量功能注释的分子标记(单核苷酸多态性(SNP)和插入缺失(InDel)),提供了关键的基因组资源信息。此外,所有这些基因组信息被用于进行初步的全基因组关联分析,并鉴定出几个与结构工程师高度关注的力学性能相关的候选基因:其垂直于纤维方向的抗拉强度(即劈裂强度)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3631/9365670/082eea475975/fpls-13-935751-g001.jpg

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