Katsube-Tanaka Tomoyuki, Monshi Fakhrul Islam
Graduate School of Agriculture, Kyoto University, Kitashirakawa, Kyoto 606-8502, Japan.
Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
Food Chem (Oxf). 2022 Jul 26;5:100127. doi: 10.1016/j.fochms.2022.100127. eCollection 2022 Dec 30.
2S albumin (2SA) is responsible for anaphylaxis following consumption of buckwheat in allergic individuals. To reduce allergen incidents, characterization of 2SA polypeptides is prerequisite, thus was analyzed in this study. Of the five 2S albumin genes ( and ), was seemingly non-functional. The g14 content was 3- and 40-fold higher than that of g11/g28 and g13, respectively. The g11/g28 were more processed to a ∼8 kDa band from a 16 kDa band than g14 in seeds, agreeing with that g11/g28 have high similarity with Fag e 8kD. Meanwhile, anti-g13 produced only a single ∼10 kDa band. Modification of g13 and domain exchange between g13 and g14 suggested that the hydrophobicity of the first domain and the nature of some amino acids in g13 contributed, at least in part, to the lower apparent molecular weight of g13 than expected. Thus, g13 might be an unexplored and noteworthy allergen.
2S清蛋白（2SA）是导致过敏个体食用荞麦后发生过敏反应的原因。为减少过敏原事件，对2SA多肽进行表征是前提条件，因此本研究对其进行了分析。在五个2S清蛋白基因（和）中，似乎无功能。g14的含量分别比g11/g28和g13高3倍和40倍。在种子中，g11/g28比g14更多地从16 kDa条带加工成约8 kDa条带，这与g11/g28与Fag e 8kD具有高度相似性一致。同时，抗g13仅产生一条约10 kDa的条带。g13的修饰以及g13和g14之间的结构域交换表明，g13中第一个结构域的疏水性和某些氨基酸的性质至少部分导致了g13的表观分子量低于预期。因此，g13可能是一种未被探索且值得关注的过敏原。
