State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, 071000 Baoding, China.
Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2208978119. doi: 10.1073/pnas.2208978119. Epub 2022 Aug 15.
Heading is one of the most important agronomic traits for Chinese cabbage crops. During the heading stage, leaf axial growth is an essential process. In the past, most genes predicted to be involved in the heading process have been based on leaf development studies in . No genes that control leaf axial growth have been mapped and cloned via forward genetics in Chinese cabbage. In this study, we characterize the inward curling mutant in ssp. and identify a mutation in the () gene by map-based cloning. OPS is involved in phloem differentiation in , a functionalization of regulating leaf curvature that is differentiated in Chinese cabbage. In the presence of brassinosteroid (BR) at the early heading stage in , the mutation of BrOPS fails to sequester brassinosteroid insensitive 2 (BrBIN2) from the nucleus, allowing BrBIN2 to phosphorylate and inactivate BrBES1, which in turn relieves the repression of and results in leaf inward curving. Taken together, the results of our findings indicate that BrOPS positively regulates BR signaling by antagonizing BrBIN2 to promote leaf epinastic growth at the early heading stage in Chinese cabbage.
标题是大白菜作物最重要的农艺性状之一。在抽薹期，叶片轴向生长是一个必不可少的过程。过去，大多数被预测参与抽薹过程的基因都是基于 的叶片发育研究。通过正向遗传学在大白菜中尚未定位和克隆控制叶片轴向生长的基因。在这项研究中，我们对 ssp. 中的内卷突变体 进行了特征描述，并通过基于图谱的克隆鉴定了 （）基因的突变。OPS 参与了 的韧皮部分化，这是一种在大白菜中分化的调节叶片曲率的功能化。在 早期抽薹阶段存在油菜素内酯（BR）时，突变的 BrOPS 无法将 BR 不敏感 2（BrBIN2）从核内隔离出来，使 BrBIN2 能够磷酸化并使 BrBES1 失活，从而解除对 的抑制作用，导致叶片内卷。总之，我们的研究结果表明，BrOPS 通过拮抗 BrBIN2 正向调节 BR 信号，促进大白菜早期抽薹阶段的 BR 信号转导，从而促进叶片的偏上生长。
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