Gao Ying, Lu Yin, Li Xiaoguang, Li Na, Zhang Xiaomeng, Su Xiangjie, Feng Daling, Liu Mengyang, Xuan Shuxin, Gu Aixia, Wang Yanhua, Chen Xueping, Zhao Jianjun, Shen Shuxing
State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding, China.
Agriculture and Rural Affairs Bureau of Xindu District, Xingtai, China.
Front Genet. 2020 Jul 23;11:773. doi: 10.3389/fgene.2020.00773. eCollection 2020.
In Chinese cabbage ( L. ssp. ), leaf adaxial-abaxial (ad-ab) polarity is tightly related to leaf incurvature, an essential factor for the formation of leafy heads. Therefore, identification of the genes responsible for leaf ad-ab polarity and studying their genetic variation may clarify the mechanism of leafy head formation. By comparing the sequences of the genes regulating leaf ad-ab polarity development in (), 41 candidate genes distributed on 10 chromosomes were found to be responsible for the establishment of ad-ab polarity in Chinese cabbage. Orthologous genes, including 10 single copies, 14 double copies, and one triple copies, were detected in the Chinese cabbage. The gene structure and conserved domain analyses showed that the number of exons of the 41 candidate genes range from one to 25, and that most genes share the conserved motifs 1, 6, and 10. Based on the 41 candidate genes, 341 simple sequence repeats (SSRs) were detected, including five replicated types: single, double, triple, quintuple, and sextuple nucleotide replications. Among these sequence repeat (SSR) loci, 323 loci were used to design 969 specific primers, and 362 primer pairs were selected randomly and evaluated using 12 Chinese cabbage accessions with different heading types. 23 primer pairs resulting with clear, polymorphic bands, combined with other 127 markers, was used to construct a linkage map by using an F population containing 214 lines derived from the hybrid of the overlapping heading Chinese cabbage "14Q-141" and the outward curling heading Chinese cabbage "14Q-279." The result showed that the sequences of markers in the genetic linkage map and the physical map was consistent in general. Our study could help to accelerate the breeding process of leafy head quality in Chinese cabbage.
在大白菜（L. ssp.）中，叶片近轴-远轴（ad-ab）极性与叶片弯曲紧密相关，而叶片弯曲是叶球形成的一个重要因素。因此，鉴定负责叶片ad-ab极性的基因并研究其遗传变异，可能会阐明叶球形成的机制。通过比较（）中调控叶片ad-ab极性发育的基因序列，发现分布在10条染色体上的41个候选基因负责大白菜ad-ab极性的建立。在大白菜中检测到直系同源基因，包括10个单拷贝、14个双拷贝和1个三拷贝。基因结构和保守结构域分析表明，41个候选基因的外显子数量从1到25不等，且大多数基因具有保守基序1、6和10。基于这41个候选基因，检测到341个简单序列重复（SSR），包括单核苷酸、双核苷酸、三核苷酸、五核苷酸和六核苷酸重复5种重复类型。在这些序列重复（SSR）位点中，323个位点用于设计969对特异性引物，随机选择362对引物，并用12个不同结球类型的大白菜种质进行评估。23对产生清晰多态性条带的引物，与其他127个标记一起，用于构建一个包含214个株系的F群体的连锁图谱，该群体来源于叠抱型大白菜“14Q-141”与外卷型大白菜“14Q-279”的杂交种。结果表明，遗传连锁图谱和物理图谱中标记的序列总体上是一致的。我们的研究有助于加快大白菜叶球品质的育种进程。
