Insight into complexation of Cd(II) and Cu(II) to fulvic acid based on feature recognition of PARAFAC combined with 2DCOS.

Fulvic acid which could govern the environmental geochemistry behavior of heavy metals is considered as the eco-friendly substances for controlling heavy metal pollutants in environment. Knowledge on the individual fulvic acid ligand is crucial to characterize the effect of fulvic acid on the migration and toxicity of metal pollutants. Herein, fulvic acid substances were analyzed by fluorescence quenching associated with parallel factor analysis (PARAFAC). Three components were identified based on PARAFAC. Furthermore, two-dimensional correlation spectroscopy (2DCOS) associated with complexation model were used to elucidate the Cd(II)- and Cu(II)-binding characteristics of the individual fulvic acid ligand. The Cd(II)- and Cu(II)-binding capability and speed of different fulvic acid ligands were revealed and theoretical guidance and technical support were provided for the practical application. The Cd(II) contaminated soil could be amended with high fulvic acid ligands A1 and Y2 containing composting products and the Cu(II) contaminated soil could be amended with high fulvic acid ligands Y1, T1 and A1 containing composting products to control the pollution and improve the soil condition. Based on these excellent results, the different fulvic acid ligands-contaminants-binding properties was characterized for the theoretical supporting of environmental pollution control.