Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China.
College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Molecules. 2022 Oct 27;27(21):7316. doi: 10.3390/molecules27217316.
Plenty of humic acid components compositions are contained in dissolved organic matter (DOM) derived from composting. Fluorescence signals were employed to characterize the changes in DOM components in the component process. In the composting process, five individual DOM fluorescence parallel factor analysis (PARAFAC) components were identified. At the end of the composting, PARAFAC component C5, which represented high humification and complex structure compounds, was detected, but the simple structure DOM PARAFAC component C1 was absent. In this study, a technique combining EEM-PARAFAC with two-dimensional correlation spectroscopy (2DCOS) further supplied detailed information about the dynamics of DOM peaks in PARAFAC components. 2DCOS results showed that the variation of the peaks in PARAFAC components was different in the composting process. The formation of a complex DOM fluorescence substance was attributed to the residues from the simple fluorescence peak degradation. The evolution of the DOM fluorescence peaks in each PARAFAC component indicated that simple structure compounds helped the formation of the complex DOM fluorescence substance in the composting process. These results revealed that EEM/PARAFAC combined with 2DCOS could be used to track the evolution of DOM PARAFAC components during the composting process.
堆肥过程中溶解有机质（DOM）中含有丰富的腐殖酸成分，利用荧光信号来表征 DOM 成分在分解过程中的变化。在堆肥过程中，鉴定出了 5 种单个 DOM 荧光平行因子分析（PARAFAC）成分。在堆肥结束时，检测到了代表高腐殖化和复杂结构化合物的 PARAFAC 成分 C5，但不存在简单结构 DOM PARAFAC 成分 C1。在这项研究中，将 EEM-PARAFAC 与二维相关光谱（2DCOS）相结合的技术进一步提供了关于 PARAFAC 成分中 DOM 峰动力学的详细信息。2DCOS 结果表明，在堆肥过程中，PARAFAC 成分中峰的变化不同。复杂 DOM 荧光物质的形成归因于简单荧光峰降解的残留物。每个 PARAFAC 成分中 DOM 荧光峰的演化表明，在堆肥过程中，简单结构化合物有助于复杂 DOM 荧光物质的形成。这些结果表明，EEM/PARAFAC 结合 2DCOS 可用于跟踪堆肥过程中 DOM PARAFAC 成分的演变。
