WWC1 是 miR-138-5p 的靶标，促进前列腺癌的进展。
WWC1, a target of miR-138-5p, facilitates the progression of prostate cancer.
机构信息
Department of Laboratory, The Third People's Hospital of Hubei Province, Wuhan 430033, Hubei, China.
Department of Laboratory, The Third People's Hospital of Hubei Province, Wuhan 430033, Hubei, China.
出版信息
Am J Med Sci. 2022 Dec;364(6):772-781. doi: 10.1016/j.amjms.2022.08.001. Epub 2022 Aug 12.
BACKGROUND
WWC1 is known to be involved in the development of cancer. Therefore, it is critical to study the molecular mechanisms and cellular roles of WWC1 in cancer therapy.
METHODS
In this study, we examined the effect of WWC1 on prostate cancer tumorigenesis and the role of miR-138-5p in prostate cancer. The expression levels of miR-138-5p and WWC1 in prostate cancer (Pca) tissues and cells were detected by real-time quantitative reverse transcription PCR and western blotting. Cell counting kit-8 and BrdU assays were performed to study cell proliferation and caspase-3 activity assay to detect apoptosis. Migration experiments were conducted to observe the movement ability of the cells.
RESULTS
The expression of WWC1 in Pca tissues or cell lines was increased, whereas miR-138-5p expression was decreased. MiR-138-5p targeted and partially neutralized the role of WWC1 in Pca cells. Moreover, reduced expression of WWC1 in Pca cell lines suppressed cell proliferation and migration and promoted apoptosis in vitro.
CONCLUSIONS
Collectively, these findings reveal a novel mechanism by which miR-138-5p negatively regulates WWC1 in Pca.
背景
已知 WWC1 参与癌症的发生。因此，研究 WWC1 在癌症治疗中的分子机制和细胞作用至关重要。
方法
在本研究中，我们研究了 WWC1 对前列腺癌肿瘤发生的影响以及 miR-138-5p 在前列腺癌中的作用。通过实时定量逆转录 PCR 和 Western blot 检测前列腺癌（Pca）组织和细胞中 miR-138-5p 和 WWC1 的表达水平。通过细胞计数试剂盒-8 和 BrdU 测定研究细胞增殖，通过 caspase-3 活性测定检测细胞凋亡。进行迁移实验观察细胞的运动能力。
结果
在 Pca 组织或细胞系中，WWC1 的表达增加，而 miR-138-5p 的表达降低。miR-138-5p 靶向并部分中和了 WWC1 在 Pca 细胞中的作用。此外，降低 Pca 细胞系中 WWC1 的表达可抑制体外细胞增殖、迁移并促进细胞凋亡。
结论
综上所述，这些发现揭示了 miR-138-5p 负调控 Pca 中 WWC1 的新机制。
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