miR-138-5p 通过靶向 FOXC1 抑制前列腺癌的进展。
MIR-138-5P inhibits the progression of prostate cancer by targeting FOXC1.
机构信息
Department of Urology, The Affiliated Shunde Hospital of Guangzhou Medical University, Foshan, China.
Department of Urology, Yangtze University, Affiliated Hospital 1, Jingzhou, China.
出版信息
Mol Genet Genomic Med. 2020 Apr;8(4):e1193. doi: 10.1002/mgg3.1193. Epub 2020 Feb 28.
BACKGROUND
Studies have suggested that micro-RNAs (miRNAs) can function as an oncogene or a tumor suppressor in cancers. However, the role of MIR-138-5P (613394) in prostate cancer (PCa) remains unclear.
METHODS
Expression level of MIR-138-5P in PCa cell lines and normal cell line was analyzed with the quantitative real-time PCR method. Cell counting kit-8 assay, colony formation assay, wound-healing assay, and transwell invasion assay were performed to analyze the biological functions of MIR-138-5P.
RESULTS
We showed MIR-138-5P expression level was significantly decreased in PCa cell lines compared with the normal cell line. Overexpression of MIR-138-5P inhibits PCa cell proliferation, colony formation, cell migration, and cell invasion in vitro. Mechanistically, we showed Forkhead box C1 (FOXC1, 601090) was a direct target for MIR-138-5P in PCa. We confirmed that overexpression of FOXC1 partially reversed the effects of MIR-138-5P on PCa cell behaviors.
CONCLUSIONS
Collectively, we showed that MIR-138-5P functions as a tumor suppressor gene in PCa via targeting FOXC1.
背景
研究表明，微小 RNA（miRNAs）可以在癌症中作为癌基因或肿瘤抑制因子发挥作用。然而，MIR-138-5P（613394）在前列腺癌（PCa）中的作用尚不清楚。
方法
采用实时定量 PCR 方法分析 PCa 细胞系和正常细胞系中 MIR-138-5P 的表达水平。通过细胞计数试剂盒-8 测定、集落形成测定、划痕愈合测定和 Transwell 侵袭测定分析 MIR-138-5P 的生物学功能。
结果
我们发现 MIR-138-5P 的表达水平在 PCa 细胞系中明显低于正常细胞系。过表达 MIR-138-5P 可抑制 PCa 细胞的体外增殖、集落形成、细胞迁移和细胞侵袭。在机制上，我们发现叉头框 C1（FOXC1，601090）是 PCa 中 MIR-138-5P 的直接靶标。我们证实过表达 FOXC1 部分逆转了 MIR-138-5P 对 PCa 细胞行为的影响。
结论
综上所述，我们表明 MIR-138-5P 通过靶向 FOXC1 在 PCa 中发挥肿瘤抑制基因的作用。
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