Key Laboratory for Applied Techonology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Key Laboratory for Applied Techonology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Fitoterapia. 2022 Oct;162:105277. doi: 10.1016/j.fitote.2022.105277. Epub 2022 Aug 12.
Six new isolates including three new alkaloids (1-3), one new secoiridoid glycoside (4) and one new 11-delactonization-secoiridoid glycoside (5), and one new organic acid (6) were identified from the leaves of Lonicera japonica, among which 1 and 2 assigned as a pair of configurational isomers possessed two oxazolidin-2-one fragments connected through NN bond. The structures were elucidated by the NMR data and ICD analysis. All the compounds (1-6) were tested for their antioxidant and hepatoprotective activities using cell models of ABAP-induced HepG cell and APAP-induced HepG cell by the MTT method. Outstandingly, compound 2 exhibited remarkable antioxidative activity with inhibitory rate of 117.2%, and compounds 2, 4, 6 exhibited significant effects with inhibitory rates of 68.1%, 69.3%, 69.2%, respectively.
从忍冬叶中分离得到 6 个新化合物，包括 3 个新生物碱（1-3）、1 个新裂环环烯醚萜苷（4）和 1 个新 11-去内酯裂环环烯醚萜苷（5），以及 1 个新有机酸（6）。其中，1 和 2 被鉴定为一对构型异构体，它们通过 NN 键连接两个恶唑烷-2-酮片段。通过 NMR 数据和 ICD 分析阐明了结构。采用 MTT 法，通过 ABAP 诱导的 HepG 细胞和 APAP 诱导的 HepG 细胞模型，对所有化合物（1-6）进行了抗氧化和保肝活性测试。值得注意的是，化合物 2 表现出显著的抗氧化活性，抑制率为 117.2%，化合物 2、4、6 表现出显著的抑制作用，抑制率分别为 68.1%、69.3%和 69.2%。
