Yoon Jong Pil, Min Seung Gi, Choi Jin-Hyun, Lee Hyun Joo, Park Kyeong Hyeon, Yoon Sung Hyuk, Kim Seong Soo, Chung Seok Won, Kim Hun-Min, Kim Dong Hyun
Department of Orthopaedic Surgery, School of Medicine, Kyungpook National University, Daegu, Korea.
Department of Bio-fibers and Materials Science, Kyungpook National University, Daegu, Korea.
Clin Shoulder Elb. 2022 Dec;25(4):296-303. doi: 10.5397/cise.2022.00976. Epub 2022 Aug 16.
A previous study reported that hyperlipidemia increases the incidence of tears in the rotator cuff tendon and affects healing after repair. The aim of our study was to compare the gene and protein expression of torn rotator cuff tendons in patients both with and without hypercholesterolemia.
Thirty patients who provided rotator cuff tendon samples were classified into either a non-hypercholesterolemia group (n=19, serum total cholesterol [TC] <200 mg/dL) and hypercholesterolemia group (n=11, serum TC ≥240 mg/dL) based on their concentrations of serum TC. The expression of various genes of interest, including COL1A1, IGF1, IL-6, MMP2, MMP3, MMP9, MMP13, TNMD, and TP53, was analyzed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, Western blot analysis was performed on the proteins encoded by interleukin (IL)-6 and TP53 that showed significantly different expression levels in real-time qRT-PCR.
Except for IGF1, the gene expression levels of IL-6, MMP2, MMP9, and TP53 were significantly higher in the hypercholesterolemic group than in the non-hypercholesterolemia group. Western blot analysis confirmed significantly higher protein levels of IL-6 and TP53 in the hypercholesterolemic group (p<0.05).
We observed an increase in inflammatory cytokine and matrix metalloproteinase (MMP) levels in hypercholesterolemic patients with rotator cuff tears. Increased levels of IL-6 and TP53 were observed at both the mRNA and protein levels. We suggest that the overexpression of IL-6 and TP53 may be a specific feature in rotator cuff disease patients with hypercholesterolemia.
先前的一项研究报告称，高脂血症会增加肩袖肌腱撕裂的发生率，并影响修复后的愈合。我们研究的目的是比较高胆固醇血症患者和非高胆固醇血症患者肩袖撕裂肌腱的基因和蛋白质表达。
根据血清总胆固醇（TC）浓度，将30例提供肩袖肌腱样本的患者分为非高胆固醇血症组（n = 19，血清TC < 200 mg/dL）和高胆固醇血症组（n = 11，血清TC≥240 mg/dL）。通过实时定量逆转录聚合酶链反应（qRT-PCR）分析包括COL1A1、IGF1、IL-6、MMP2、MMP3、MMP9、MMP13、TNMD和TP53在内的各种感兴趣基因的表达。此外，对在实时qRT-PCR中显示出显著不同表达水平的白细胞介素（IL）-6和TP53编码的蛋白质进行了蛋白质印迹分析。
除IGF1外，高胆固醇血症组中IL-6、MMP2、MMP9和TP53的基因表达水平显著高于非高胆固醇血症组。蛋白质印迹分析证实高胆固醇血症组中IL-6和TP53的蛋白质水平显著更高（p < 0.05）。
我们观察到高胆固醇血症伴肩袖撕裂患者的炎性细胞因子和基质金属蛋白酶（MMP）水平升高。在mRNA和蛋白质水平均观察到IL-6和TP53水平升高。我们认为IL-6和TP53的过表达可能是高胆固醇血症肩袖疾病患者的一个特征。
