Laboratoire de Parasitologie, University Manouba, Institution de la Recherche et de l'Enseignement Supérieur Agricoles, École Nationale de Médecine Vétérinaire de Sidi Thabet, Sidi Thabet, Tunisia.
Laboratoire d'anatomie Pathologique, École Nationale de Médecine Vétérinaire de Sidi Thabet, Sidi Thabet, Tunisia.
Vet Med Sci. 2022 Sep;8(5):2241-2247. doi: 10.1002/vms3.901. Epub 2022 Aug 16.
Neospora caninum has been documented to infect most domestic wildlife but is known to primarily infect dogs and cattle and is considered an important cause of abortion in camels.
The aim of this study was to estimate the molecular detection of Neospora caninum in tissues of naturally infected camelids.
Brain, tongue (bottom and tip) and masseter muscles from 35 slaughtered camelids from Tataouine and Médenine regions were collected (n = 140 samples). PCR was used to amplify and detect N. caninum DNA in tissues samples followed by sequencing of some PCR products. A phylogenetic tree was then constructed to compare the partial sequences of the ITS1 gene with GenBank sequences. Histopathology examination was used to detect Neospora spp. cysts, but no lesions were observed.
The overall molecular detection of N. caninum in camelids was 34.3% (12/35). The highest molecular detection of N. caninum was recorded in animals of more than 3 years old (6/9) and in animals aged between 1 and 3 years old (4/12). Whilst, the lowest molecular detection (2/14) was observed in animals 1 year or younger (p = 0.035). There were no significant differences in molecular detection of N. caninum according to both locality and gender (p > 0.05). Similarly, there was no difference of prevalence between different anatomical locations. Comparison of the partial sequences of the ITS1 gene revealed 100-95.5% similarity among our N. caninum amplicon (MW551566) and those deposited in GenBank.
These results highlight the presence of a risk infection by N. caninum in camels. For preventing N. caninum infection further studies are needed to improve our knowledge about the epidemiology of neosporosis in North Africa.
已记录有neospora caninum 感染大多数家养野生动物，但已知其主要感染犬和牛，被认为是骆驼流产的重要原因。
本研究旨在估计天然感染骆驼组织中neospora caninum 的分子检测。
从 Tataouine 和Médenine 地区屠宰的 35 只骆驼中采集脑、舌（底部和尖端）和咬肌肌肉（n = 140 个样本）。使用 PCR 扩增和检测组织样本中的 N. caninum DNA，然后对一些 PCR 产物进行测序。然后构建系统发育树，将 ITS1 基因的部分序列与 GenBank 序列进行比较。组织病理学检查用于检测neospora spp. 囊肿，但未观察到病变。
骆驼中 N. caninum 的总体分子检测率为 34.3%（12/35）。在年龄超过 3 岁的动物（6/9）和 1 至 3 岁的动物（4/12）中记录到 N. caninum 的最高分子检测率。而在 1 岁或以下的动物（2/14）中观察到的最低分子检测率（p = 0.035）。根据所在地和性别，N. caninum 的分子检测率没有显著差异（p > 0.05）。同样，不同解剖部位的流行率没有差异。对 ITS1 基因的部分序列进行比较，显示我们的 N. caninum 扩增子（MW551566）与 GenBank 中存储的扩增子之间具有 100-95.5%的相似性。
这些结果突出表明骆驼存在 N. caninum 感染的风险。为了预防 N. caninum 感染，需要进一步研究来提高我们对北非新生隐球菌病流行病学的认识。
