UC Davis Eyepod Imaging Laboratory, University of California Davis, Davis, CA, USA.
Department of Ophthalmology & Vision Science, University of California Davis, Sacramento, CA, USA.
Transl Vis Sci Technol. 2022 Aug 1;11(8):11. doi: 10.1167/tvst.11.8.11.
To perform in vivo evaluation of the structural morphology and vascular plexuses of the neurosensory retina and choroid across vertebrate species using swept-source optical coherence tomography (SS-OCT) and SS-OCT angiography (SS-OCTA) imaging.
A custom-built SS-OCT system with an incorporated flexible imaging arm was used to acquire the three-dimensional (3D) retinal OCT and vascular OCTA data of five different vertebrates: a mouse (C57BL/6J), a rat (Long Evans), a gray short-tailed opossum (Monodelphis domestica), a white sturgeon (Acipenser transmontanus), and a great horned owl (Bubo virginianus).
In vivo structural morphology of the retina and choroid, as well as en face OCTA images of retinal and choroidal vasculature of all species were generated. The retinal morphology and vascular plexuses were similar between rat and mouse, whereas distinct choroidal and paired superficial vessels were observed in the opossum retina. The retinal and vascular structure of the sturgeon, as well as the pecten oculi and overlying the avascular and choroidal vasculature in the owl retina are reported in vivo.
A high-quality two-dimensional and 3D in vivo visualization of the retinal structures and en face visualization of the retina and choroidal vascular plexus of vertebrates was possible. Our studies affirm that SS-OCT and SS-OCTA are viable methods for evaluating the in vivo retinal and choroidal structure across terrestrial, aquatic, and aerial vertebrates.
In vivo characterization of retinal morphology and vasculature plexus of multiple species using SS-OCT and SS-OCTA imaging can increase the pool of species available as models of human retinal diseases.
利用扫频源光学相干断层扫描（SS-OCT）和 SS-OCT 血管成像（SS-OCTA）成像，对不同脊椎动物的神经感觉视网膜和脉络膜的结构形态和血管丛进行体内评估。
使用带有内置柔性成像臂的定制 SS-OCT 系统，获取 5 种不同脊椎动物的三维（3D）视网膜 OCT 和血管 OCTA 数据：小鼠（C57BL/6J）、大鼠（Long Evans）、短尾灰负鼠（Monodelphis domestica）、白鲟（Acipenser transmontanus）和大角鸮（Bubo virginianus）。
生成了所有物种的视网膜和脉络膜的体内结构形态以及视网膜和脉络膜血管的面 OCTA 图像。大鼠和小鼠的视网膜形态和血管丛相似，而负鼠的视网膜则观察到明显的脉络膜和成对的浅层血管。报道了鲟鱼的视网膜和血管结构，以及鸮的眼梳和无血管脉络膜血管上方的结构。
可以对脊椎动物的视网膜结构进行高质量的二维和 3D 体内可视化，并对面成像视网膜和脉络膜血管丛。我们的研究证实，SS-OCT 和 SS-OCTA 是评估陆生、水生和空中脊椎动物体内视网膜和脉络膜结构的可行方法。
顾泽宇
