Opt Lett. 2024 Aug 15;49(16):4630-4633. doi: 10.1364/OL.531116.
A full-field swept-source optical coherence tomography (FF-SS-OCT) for in vivo murine retinal imaging is demonstrated. The on-axis FF-SS-OCT system was built in a Mach-Zehnder interferometer configuration employing a tunable laser source with an adjustable sweep rate and sweep range in conjunction with a fast 2D-CMOS camera. A large field retinal (coherent) illumination was accomplished using an imaging interface comprised of a short-focal length imaging lens and a contact lens. The magnification between the camera and retina (spatial sampling) was appropriately chosen to record the microscopic structural features of the retina in the image. A pupil stop was employed in the detection path to reject unwanted backscattering from the mouse eye and other sources and limit aberrations distorting the retinal images. In vivo mouse retinal imaging was performed at a sweep rate of 150 Hz to acquire volumes unaffected by the system vibrations, which predominated at lower frequencies. Operating the FF-SS-OCT at this speed yielded an effective axial scan rate of 20 million A-scans/s and a field of view of 820 × 410 µm (24.12° × 12.06°). High-quality retinal B-scans and enface images of the retina were obtained with the SS-FF-OCT, revealing all major retinal layers and vascular plexuses.
我们展示了一种用于活体鼠视网膜成像的全场扫频光学相干断层扫描（FF-SS-OCT）系统。该系统采用马赫-曾德尔干涉仪结构，配备了可调谐激光源，可调节扫频速率和扫频范围，并结合了高速 2D-CMOS 相机。通过使用短焦距成像透镜和接触透镜组成的成像接口，实现了大视场视网膜（相干）照明。在探测路径中使用光瞳阻挡器来排除来自小鼠眼睛和其他来源的不需要的后向散射，并限制扭曲视网膜图像的像差。在 150Hz 的扫频速率下对活体鼠的视网膜进行成像，以获取不受系统振动影响的体积，系统振动在较低频率时占主导地位。以该速度运行 FF-SS-OCT 可得到 2000 万次/秒的有效轴向扫描速率和 820×410µm 的视野（24.12°×12.06°）。通过 SS-FF-OCT 获得了高质量的视网膜 B 扫描和视网膜的层面图像，揭示了所有主要的视网膜层和血管丛。
