Department of Medical Physiology, Faculty of Medicine, Tanta University, Tanta, Egypt.
Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt.
Pflugers Arch. 2022 Oct;474(10):1107-1119. doi: 10.1007/s00424-022-02739-y. Epub 2022 Aug 16.
Obesity is linked to reproductive disorders. Novel neuropeptide phoenixin demonstrated many therapeutic actions. In this study, we aim to evaluate phoenixin's potential effect in obesity-induced infertility through modulating mitochondrial dynamics. Ninety adult female rats were divided to 4 groups: (I), fed with normal pellet diet; (II), given phoenixin; (III), fed with high-fat diet. Rats that developed obesity and infertility were divided to 2 groups: (III-A), received no further treatment; (III-B), given phoenixin. Our results showed that phoenixin treatment in obese infertile rats significantly decreased serum levels of insulin and testosterone and ovarian levels of dynamin-related protein1(Drp1),reactive oxygen species ROS, TNF-α, MDA, and caspase-3. Phoenixin treatment also significantly increased serum estrogen progesterone, LH, and FSH together with ovarian levels of GnRH receptor (GnRHR), mitofusin2(Mfn2), mitochondrial transmembrane potential (ΔΨm), and electron transport chain (ETC) complex-I significantly when compared with obese group. Ovarian histopathological changes were similarly improved by phoenixin. Our data demonstrate phoenixin's role in improving obesity-induced infertility.
肥胖与生殖障碍有关。新型神经肽凤凰素表现出许多治疗作用。在这项研究中，我们旨在通过调节线粒体动力学来评估凤凰素在肥胖引起的不孕中的潜在作用。将 90 只成年雌性大鼠分为 4 组：（I），喂以正常颗粒饮食；（II），给予凤凰素；（III），给予高脂肪饮食。肥胖和不孕的大鼠分为 2 组：（III-A），不进行进一步治疗；（III-B），给予凤凰素。我们的结果表明，肥胖不孕大鼠给予凤凰素治疗后，血清胰岛素和睾酮水平以及卵巢中动力相关蛋白 1（Drp1）、活性氧（ROS）、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）和半胱天冬酶-3（caspase-3）水平显著降低。与肥胖组相比，凤凰素治疗还显著增加了血清雌激素、孕激素、LH 和 FSH 水平，以及卵巢中的促性腺激素释放激素受体（GnRHR）、线粒体融合蛋白 2（Mfn2）、线粒体跨膜电位（ΔΨm）和电子传递链（ETC）复合物-I 水平。凤凰素还改善了卵巢的组织病理学变化。我们的数据表明凤凰素在改善肥胖引起的不孕中的作用。
