Ritzinger Mitchell G, Smith Kevin P, Case Austin J, Wodarek Joseph R, Dill-Macky Ruth, Curland Rebecca D, Steffenson Brian J
Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.
Department of Agronomy, University of Minnesota, St. Paul, MN 55108.
Plant Dis. 2023 Mar;107(3):802-808. doi: 10.1094/PDIS-04-22-0751-RE. Epub 2023 Mar 7.
Bacterial leaf streak (BLS) is a sporadic yet damaging disease of cereals that is growing in importance across the Upper Midwest production region. In barley ( ssp. ), this disease is caused primarily by the bacterium pv. . Accessions resistant to BLS have been reported in past studies, but few have been rigorously validated in the field. To identify accessions carrying diverse resistance alleles to BLS, a largescale germplasm screening study was undertaken against strain CIX95 of pv. in St. Paul and Crookston, Minnesota, in 2020 and 2021. The germplasm screened was diverse and included adapted breeding lines from two improvement programs, two landrace panels (one global and one from Ethiopia/Eritrea), introgression lines from wild barley ( ssp. ) in the genetic background of barley cultivar 'Rasmusson', and an assemblage of accessions previously reported to carry BLS resistance. Of the 2,094 accessions evaluated in this study, 32 (1.5%) exhibited a consistently high level of resistance across locations and years and had heading dates similar to standard cultivars grown in the region. Accessions resistant to BLS were identified from all germplasm panels tested, providing genetically diverse sources for barley improvement programs focused on breeding for resistance to this important bacterial disease.
细菌性条斑病是一种偶发性但具有破坏性的谷物病害，在中西部上游产区的重要性日益增加。在大麦（亚种）中，这种病害主要由细菌引起。过去的研究报道了对细菌性条斑病具有抗性的种质，但很少在田间进行严格验证。为了鉴定携带不同抗性等位基因的种质，2020年和2021年在明尼苏达州圣保罗和克鲁克斯顿针对菌株CIX95进行了大规模种质筛选研究。筛选的种质具有多样性，包括来自两个改良计划的适应性育种系、两个地方品种群体（一个全球群体和一个来自埃塞俄比亚/厄立特里亚）、大麦品种“拉斯穆森”遗传背景下野生大麦（亚种）的渐渗系，以及先前报道携带细菌性条斑病抗性的种质组合。在本研究评估的2094份种质中，32份（1.5%）在不同地点和年份均表现出持续高水平的抗性，抽穗期与该地区种植的标准品种相似。从所有测试的种质群体中都鉴定出了对细菌性条斑病具有抗性的种质，为专注于培育抗这种重要细菌病害的大麦改良计划提供了遗传多样性来源。
