Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108.
Phytopathology. 2023 Nov;113(11):2119-2126. doi: 10.1094/PHYTO-10-22-0402-SA. Epub 2023 Nov 17.
Bacterial leaf streak (BLS), caused chiefly by the pathogen pv. , is becoming an increasingly important foliar disease of barley in the Upper Midwest. The deployment of resistant cultivars is the most economical and practical method of control. To identify sources of BLS resistance, we evaluated two panels of breeding lines from the University of Minnesota (UMN) and Anheuser-Busch InBev (ABI) barley improvement programs for reaction to strain CIX95 in the field at St. Paul and Crookston, MN, in 2020 and 2021. The percentage of resistant lines in the UMN and ABI panels with mid-season maturity was 1.8% (6 of 333 lines) and 5.2% (13 of 251 lines), respectively. Both panels were genotyped with the barley 50K iSelect SNP array, and then a genome-wide association study was performed. A single, highly significant association was identified for BLS resistance on chromosome 6H in the UMN panel. This association was also identified in the ABI panel. Seven other significant associations were detected in the ABI panel: two each on chromosomes 1H, 2H, and 3H and one on chromosome 5H. Of the eight associations identified in the panels, five were novel. The discovery of resistance in elite breeding lines will hasten the time needed to develop and release a BLS-resistant cultivar.
细菌叶斑病（BLS）主要由病原体 pv. 引起，正在成为中西部大地上大麦日益重要的叶部病害。利用抗性品种是控制该病最经济、最实用的方法。为了鉴定 BLS 抗性的来源，我们评估了来自明尼苏达大学（UMN）和安海斯-布希英博（ABI）大麦改良项目的两个育成品系群体，以评估它们在圣保罗和克罗克顿（MN）田间对 CIX95 菌株的反应，分别在 2020 年和 2021 年进行。UMN 和 ABI 群体中具有中熟期的抗性品系比例分别为 1.8%（333 个品系中的 6 个）和 5.2%（251 个品系中的 13 个）。这两个群体都用大麦 50K iSelect SNP 阵列进行了基因型分析，然后进行了全基因组关联研究。在 UMN 群体中鉴定到与 6H 染色体上的 BLS 抗性高度显著相关的单个关联。在 ABI 群体中也发现了该关联。在 ABI 群体中还检测到另外 7 个显著关联：每个染色体 1H、2H 和 3H 上各有 2 个，染色体 5H 上有 1 个。在这两个群体中鉴定到的 8 个关联中，有 5 个是新的。在优良育成品系中发现抗性，将加速开发和释放 BLS 抗性品种所需的时间。
