McNeil M, Tsang A Y, McClatchy J K, Stewart C, Jardine I, Brennan P J
J Bacteriol. 1987 Jul;169(7):3312-20. doi: 10.1128/jb.169.7.3312-3320.1987.
Mycobacterium malmoense is the latest of a roster of atypical mycobacteria implicated in pulmonary infections. Yet it lacks recognizable phenotypic features to allow its ready identification. Some 23 clinical isolates of M. malmoense were examined for homologous seroagglutination reactions and characteristic surface antigens. One group showed concordant agglutination interreactions and an identical spectrum of glycolipids and are regarded as M. malmoense sensu stricto. The glycolipids are of the newly found, trehalose-containing lipooligosaccharide class. De-O-acylation followed by high-pressure liquid chromatography revealed one major and several minor oligosaccharides. Partial acidic cleavage to release glycosidically linked trehalose, alpha-mannosidase digestion to demonstrate the presence of a non-reducing-end mannobiose, perdeuteriomethylation, partial acid hydrolysis, reduction, and O ethylation, combined with 1H nuclear magnetic resonance and electron impact and fast-atom bombardment mass spectrometry revealed the structure of the major oligosaccharide as alpha-D-Manp-(1----3) -alpha-D-Manp-(1----[2-alpha-L-Rhap-(1--]4--3)-alpha-L-Rh ap- (1----3)-alpha-D-Glcp-(1----1)-alpha-D-Glcp, in which two of the 2-alpha-L-Rhap residues are O methylated at C-3. (Man, mannose; Rha, rhamnose; Glc, glucose; p, pyranosyl). The structures of the minor oligosaccharides were also determined; they differ at the distal nonreducing end. The dominant oligosaccharide was acylated by octanoate, 2-methyleicosanoate, and 2,4-dimethylpentacosanoate to yield the major species-specific surface antigen of M. malmoense, which we regard as the most characteristic feature of the pathogen.
马尔默分枝杆菌是一系列与肺部感染有关的非典型分枝杆菌中最新发现的一种。然而，它缺乏可识别的表型特征，难以进行快速鉴定。对约23株马尔默分枝杆菌临床分离株进行了同源血清凝集反应和特征性表面抗原检测。其中一组显示出一致的凝集相互反应以及相同的糖脂谱，被视为严格意义上的马尔默分枝杆菌。这些糖脂属于新发现的含海藻糖的脂寡糖类别。脱O - 酰化后进行高压液相色谱分析，揭示出一种主要的和几种次要的寡糖。部分酸性裂解以释放糖苷键连接的海藻糖，α - 甘露糖苷酶消化以证明存在非还原端甘露二糖，全氘代甲基化、部分酸水解、还原和O - 乙基化，结合1H核磁共振以及电子轰击和快原子轰击质谱分析，揭示出主要寡糖的结构为α - D - 甘露糖基 - (1→3) - α - D - 甘露糖基 - (1→[2 - α - L - 鼠李糖基 - (1→]4→3) - α - L - 鼠李糖基 - (1→3) - α - D - 葡萄糖吡喃糖基 - (1→1) - α - D - 葡萄糖吡喃糖基），其中两个2 - α - L - 鼠李糖基残基在C - 3位被O - 甲基化。（Man，甘露糖；Rha，鼠李糖；Glc，葡萄糖；p，吡喃糖基）。还确定了次要寡糖的结构；它们在远端非还原端有所不同。主要的寡糖被辛酸、2 - 甲基二十烷酸和2,4 - 二甲基二十五烷酸酰化，产生马尔默分枝杆菌主要的种特异性表面抗原，我们将其视为该病原体最具特征性的特征。
