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产氨短杆菌ATCC 6872中的锰转运

Manganese transport in Brevibacterium ammoniagenes ATCC 6872.

作者信息

Schmid J, Auling G

出版信息

J Bacteriol. 1987 Jul;169(7):3385-7. doi: 10.1128/jb.169.7.3385-3387.1987.

Abstract

Uptake of manganese by Brevibacterium ammoniagenes ATCC 6872 was energy dependent and obeyed saturation kinetics (Km = 0.65 microM; Vmax = 0.12 mumol/min per g [dry weight]). Uptake showed optima at 27 degrees C and pH 9.5. 54Mn2+ accumulated by the cells was released by treatment with toluene or by exchange for unlabeled manganese ions, via an energy-dependent process. Co2+, Fe2+, Cd2+, and Zn2+ inhibited manganese uptake. Inhibition by Cd2+ and Zn2+ was competitive (Ki = 0.15 microM Cd2+ and 1.2 microM Zn2+). Experiments with 65Zn2+ provided no evidence for Zn2+ uptake via the Mn2+ transport system.

摘要

产氨短杆菌ATCC 6872对锰的摄取是能量依赖性的,并遵循饱和动力学(Km = 0.65微摩尔;Vmax = 0.12微摩尔/分钟每克[干重])。摄取在27℃和pH 9.5时表现出最佳状态。细胞积累的54Mn2+通过用甲苯处理或通过与未标记的锰离子交换而释放,这是一个能量依赖性过程。Co2+、Fe2+、Cd2+和Zn2+抑制锰的摄取。Cd2+和Zn2+的抑制是竞争性的(Ki = 0.15微摩尔Cd2+和1.2微摩尔Zn2+)。用65Zn2+进行的实验没有提供通过Mn2+转运系统摄取Zn2+的证据。

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