School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, Liaoning 116029, China.
School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, Liaoning 116029, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Dec 15;283:121736. doi: 10.1016/j.saa.2022.121736. Epub 2022 Aug 12.
Based on the fluorescence resonance energy transfer (FRET), a ratiometric fluorescent probe (NQ) was successfully designed and synthesized, in which quinolinone moiety was selected as the energy donor and naphthalimide block as the energy acceptor. NQ has a super large Stokes shift (231 nm) and a big quantum yield (0.463). Compared with previously reported probes with similar recognition sites, NQ can high sensitively and selectively recognize ClO with a much low limit of detection (LOD = 21 nM) and extremely rapid response time (20 s). NQ has a strong anti-interference effect and a color change in the solution which can be seen by the "naked eye". Moreover, NQ can be applied to detect ClO in real water samples and living cells imaging.
基于荧光共振能量转移（FRET）原理，成功设计并合成了一种比率型荧光探针（NQ），其中喹喔啉部分被选为能量供体，而萘酰亚胺部分则被选为能量受体。NQ 具有超大的斯托克斯位移（231nm）和大的量子产率（0.463）。与具有类似识别位点的先前报道的探针相比，NQ 可以高灵敏度和选择性地识别 ClO，其检测限（LOD=21nM）非常低，响应时间极快（20s）。NQ 具有很强的抗干扰能力，并且在溶液中的颜色变化可以用“肉眼”观察到。此外，NQ 可以用于检测实际水样和活细胞成像中的 ClO。
