College of Chemical and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.
Hebei Lansheng Biotech Co., Ltd., Shijiazhuang 052260, China.
Molecules. 2023 May 5;28(9):3896. doi: 10.3390/molecules28093896.
A novel dual-response fluorescence probe () was developed by using a fluorescence priming strategy for quantitative monitoring and visualization of hydrazine (NH) and hypochlorite (ClO). With the addition of NH/ClO, the cleavage reaction of C=C bond initiated by NH/ClO was transformed into corresponding hydrazone and aldehyde derivatives, inducing the probe appeared a fluorescence "off-on" response, which was verified by DFT calculation. HRMS spectra were also conducted to confirm the sensitive mechanism of to NH and ClO. The probe had an obvious fluorescence response to NH and ClO, which caused a significant color change in unprotected eyes. In addition, the detection limits of for NH and ClO were 27 nM and 34 nM, respectively. Interference tests showed that other competitive analytes could hardly interfere with the detection of NH and ClO in a complex environment. In order to realize the point-of-care detection of NH and ClO, an @hydrogel test kit combined with a portable smartphone was developed. Furthermore, the portable test kit has been applied to the detection of NH and ClO in a real-world environment and food samples, and a series of good results have been achieved. Attractively, we demonstrated that @hydrogel was successfully applied as an encryption ink in the field of information security. Finally, the probe can also be used to monitor and distinguish NH and ClO in living cells, exhibiting excellent biocompatibility and low cytotoxicity.
一种新型的双重响应荧光探针（）被开发出来，采用荧光引发策略，用于定量监测和可视化肼（NH）和次氯酸盐（ClO）。随着 NH/ClO 的加入，由 NH/ClO 引发的 C=C 键的断裂反应被转化为相应的腙和醛衍生物，导致探针 出现荧光“关-开”响应，这通过 DFT 计算得到了验证。高分辨率质谱（HRMS）谱也被用来证实 对 NH 和 ClO 的敏感机制。探针 对 NH 和 ClO 有明显的荧光响应，导致在未受保护的眼睛中发生明显的颜色变化。此外，探针 对 NH 和 ClO 的检测限分别为 27 nM 和 34 nM。干扰测试表明，在复杂环境中，其他竞争性分析物几乎不会干扰 NH 和 ClO 的检测。为了实现 NH 和 ClO 的即时检测，开发了一种结合便携式智能手机的 @hydrogel 测试试剂盒。此外，该便携式测试试剂盒已应用于实际环境和食品样本中 NH 和 ClO 的检测，并取得了一系列良好的结果。吸引人的是，我们证明了 @hydrogel 成功地应用于信息安全领域的加密墨水。最后，该探针还可用于监测和区分活细胞中的 NH 和 ClO，表现出优异的生物相容性和低细胞毒性。
